Rapid Matrix-Assisted Laser Desorption/Ionization
Time-of-Flight Mass Spectrometry Imaging with Scanning
Desorption Laser Beam

J 2014

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

BEDNAŘÍK, Antonín, Pavel KUBA, Eugene MOSKOVETS, Iva TOMALOVÁ, Pavel KRÁSENSKÝ et. al.

Basic information

Original name

Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam

Authors

BEDNAŘÍK, Antonín (203 Czech Republic, belonging to the institution), Pavel KUBA (203 Czech Republic), Eugene MOSKOVETS (643 Russian Federation), Iva TOMALOVÁ (203 Czech Republic, belonging to the institution), Pavel KRÁSENSKÝ (203 Czech Republic, belonging to the institution), Pavel HOUŠKA (203 Czech Republic) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytical Chemistry, Washington, American Chemical Society, 2014, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.636

RIV identification code

RIV/00216224:14740/14:00073489

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/ac402823n

UT WoS

000330204500004

Keywords in English

Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput

Abstract

V originále

This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP206/12/0538, research and development project

Name: Nové strategie úpravy vzorků pro desorpční hmotnostní spektrometrii

Investor: Czech Science Foundation

GCP206/10/J012, research and development project

Name: Analýza biomolekul hmotnostní spektrometrií s laserovou desorpcí/ionizací za účasti nanomateriálů

Investor: Czech Science Foundation

Citovat

BEDNAŘÍK, Antonín, Pavel KUBA, Eugene MOSKOVETS, Iva TOMALOVÁ, Pavel KRÁSENSKÝ, Pavel HOUŠKA and Jan PREISLER. Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam. Analytical Chemistry. Washington: American Chemical Society, 2014, vol. 86, No 2, p. 982-986. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/ac402823n.

@article{1164080,
   author = {Bednařík, Antonín and Kuba, Pavel and Moskovets, Eugene and Tomalová, Iva and Krásenský, Pavel and Houška, Pavel and Preisler, Jan},
   article_location = {Washington},
   article_number = {2},
   doi = {http://dx.doi.org/10.1021/ac402823n},
   keywords = {Mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam},
   url = {http://pubs.acs.org/doi/abs/10.1021/ac402823n},
   volume = {86},
   year = {2014}
}

TY  - JOUR
ID  - 1164080
AU  - Bednařík, Antonín - Kuba, Pavel - Moskovets, Eugene - Tomalová, Iva - Krásenský, Pavel - Houška, Pavel - Preisler, Jan
PY  - 2014
TI  - Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
JF  - Analytical Chemistry
VL  - 86
IS  - 2
SP  - 982-986
EP  - 982-986
PB  - American Chemical Society
SN  - 00032700
KW  - Mass spectrometry imaging
KW  - laser beam scanning
KW  - MALDI
KW  - TOF
KW  - high-throughput
UR  - http://pubs.acs.org/doi/abs/10.1021/ac402823n
L2  - http://pubs.acs.org/doi/abs/10.1021/ac402823n
N2  - This work shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in 11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
ER  -

BEDNAŘÍK, Antonín, Pavel KUBA, Eugene MOSKOVETS, Iva TOMALOVÁ, Pavel KRÁSENSKÝ, Pavel HOUŠKA and Jan PREISLER. Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam. \textit{Analytical Chemistry}. Washington: American Chemical Society, 2014, vol.~86, No~2, p.~982-986. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/ac402823n.

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