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@inbook{1164717,
author = {Lysák, Martin and Mandáková, Terezie},
address = {New York},
booktitle = {Plant Meiosis: Methods and Protocols},
doi = {http://dx.doi.org/10.1007/978-1-62703-333-6_2},
editor = {Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan},
keywords = {Chromosome painting; Fluorescence in situ hybridization; BAC FISH; Pachytene chromosomes; DNA labeling},
howpublished = {tištěná verze "print"},
language = {eng},
location = {New York},
isbn = {978-1-62703-332-9},
pages = {13-24},
publisher = {Humana Press},
title = {Analysis of Plant Meiotic Chromosomes by Chromosome Painting},
url = {http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6},
year = {2013}
}
TY - CHAP
ID - 1164717
AU - Lysák, Martin - Mandáková, Terezie
PY - 2013
TI - Analysis of Plant Meiotic Chromosomes by Chromosome Painting
VL - Part 1
PB - Humana Press
CY - New York
SN - 9781627033329
KW - Chromosome painting
KW - Fluorescence in situ hybridization
KW - BAC FISH
KW - Pachytene chromosomes
KW - DNA labeling
UR - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6
L2 - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6
N2 - Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.
ER -
LYSÁK, Martin and Terezie MANDÁKOVÁ. Analysis of Plant Meiotic Chromosomes by Chromosome Painting. In Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan. \textit{Plant Meiosis: Methods and Protocols}. New York: Humana Press, 2013, p.~13-24. Part 1. ISBN~978-1-62703-332-9. Available from: https://dx.doi.org/10.1007/978-1-62703-333-6\_{}2.
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