Analysis of Plant Meiotic Chromosomes by Chromosome Painting

C 2013

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

LYSÁK, Martin and Terezie MANDÁKOVÁ

Basic information

Original name

Analysis of Plant Meiotic Chromosomes by Chromosome Painting

Authors

LYSÁK, Martin (203 Czech Republic, guarantor, belonging to the institution) and Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution)

Edition

New York, Plant Meiosis: Methods and Protocols, p. 13-24, 12 pp. Part 1, 2013

Publisher

Humana Press

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14740/13:00065831

Organization unit

Central European Institute of Technology

ISBN

978-1-62703-332-9

DOI

http://dx.doi.org/10.1007/978-1-62703-333-6_2

Keywords in English

Chromosome painting; Fluorescence in situ hybridization; BAC FISH; Pachytene chromosomes; DNA labeling

Abstract

V originále

Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.

Links

GAP501/10/1014, research and development project

Name: Evoluce genomu alopolyploidních řeřišnic (Cardamine) rozdílného fylogenetického stáří

Investor: Czech Science Foundation

IAA601630902, research and development project

Name: Evoluce chromosomů brukvovitých (Brassicaceae) analyzována pomocí komparativního chromosomálního paintingu

Investor: Academy of Sciences of the Czech Republic, Chromosome evolution in crucifers (Brassicaceae) revealed by comparative chromosome painting

Citovat

LYSÁK, Martin and Terezie MANDÁKOVÁ. Analysis of Plant Meiotic Chromosomes by Chromosome Painting. In Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan. Plant Meiosis: Methods and Protocols. New York: Humana Press, 2013, p. 13-24. Part 1. ISBN 978-1-62703-332-9. Available from: https://dx.doi.org/10.1007/978-1-62703-333-6_2.

@inbook{1164717,
   author = {Lysák, Martin and Mandáková, Terezie},
   address = {New York},
   booktitle = {Plant Meiosis: Methods and Protocols},
   doi = {http://dx.doi.org/10.1007/978-1-62703-333-6_2},
   editor = {Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan},
   keywords = {Chromosome painting; Fluorescence in situ hybridization; BAC FISH; Pachytene chromosomes; DNA labeling},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {New York},
   isbn = {978-1-62703-332-9},
   pages = {13-24},
   publisher = {Humana Press},
   title = {Analysis of Plant Meiotic Chromosomes by Chromosome Painting},
   url = {http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6},
   year = {2013}
}

TY  - CHAP
ID  - 1164717
AU  - Lysák, Martin - Mandáková, Terezie
PY  - 2013
TI  - Analysis of Plant Meiotic Chromosomes by Chromosome Painting
VL  - Part 1
PB  - Humana Press
CY  - New York
SN  - 9781627033329
KW  - Chromosome painting
KW  - Fluorescence in situ hybridization
KW  - BAC FISH
KW  - Pachytene chromosomes
KW  - DNA labeling
UR  - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6
L2  - http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-333-6
N2  - Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.
ER  -

LYSÁK, Martin and Terezie MANDÁKOVÁ. Analysis of Plant Meiotic Chromosomes by Chromosome Painting. In Pawlowski Wojciech P.; Grelon Mathidle; Armstrong Susan. \textit{Plant Meiosis: Methods and Protocols}. New York: Humana Press, 2013, p.~13-24. Part 1. ISBN~978-1-62703-332-9. Available from: https://dx.doi.org/10.1007/978-1-62703-333-6\_{}2.

Detailed Information on Publication Record