KOVÁČIK, Lubomír, Sami KEREÏCHE, Johanna HÖÖG, Pavel JŮDA, Pavel MATULA and Ivan RAŠKA. A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions. Journal of Structural Biology. Academic Press, 2014, vol. 186, No 1, p. 141-152. ISSN 1047-8477. Available from: https://dx.doi.org/10.1016/j.jsb.2014.02.004.
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Basic information
Original name A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions
Authors KOVÁČIK, Lubomír (203 Czech Republic), Sami KEREÏCHE (250 France), Johanna HÖÖG (752 Sweden), Pavel JŮDA (203 Czech Republic), Pavel MATULA (203 Czech Republic, guarantor, belonging to the institution) and Ivan RAŠKA (203 Czech Republic).
Edition Journal of Structural Biology, Academic Press, 2014, 1047-8477.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.231
RIV identification code RIV/00216224:14330/14:00073497
Organization unit Faculty of Informatics
Doi http://dx.doi.org/10.1016/j.jsb.2014.02.004
UT WoS 000334570200015
Keywords in English Electron tomography; Missing wedge; Missing wedge artefacts; Single-axis tilting
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Pavel Matula, Ph.D., učo 2927. Changed: 28/4/2015 13:32.
Abstract
he limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with its impulse response, which gives rise to the well-known missing wedge artefacts in 3D reconstructions. Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology. Therefore we have designed a simple Fourier angular filter that effectively suppresses the ray artefacts in the single-axis tilting projection acquisition scheme, making single-axis tomographic reconstructions easier to interpret in particular at low signal-to-noise ratio in acquired projections. The proposed filter can be easily incorporated into current electron tomographic reconstruction schemes.
Links
GBP302/12/G157, research and development projectName: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
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