NODZYNSKI, Tomasz, Mugurel I FERARU, Sibylle HIRSCH, Riet DE RYCKE, Claudiu NICULAES, Wout BOERJAN, Jelle VAN LEENE, Geert DE JAEGER, Steffen VANNESTE and Jiří FRIML. Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis. Molecular Plant. Oxford: Oxford University Press, 2013, vol. 6, No 6, p. 1849-1862. ISSN 1674-2052. doi:10.1093/mp/sst044.
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Basic information
Original name Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis
Authors NODZYNSKI, Tomasz (616 Poland, belonging to the institution), Mugurel I FERARU (56 Belgium), Sibylle HIRSCH (56 Belgium), Riet DE RYCKE (56 Belgium), Claudiu NICULAES (56 Belgium), Wout BOERJAN (56 Belgium), Jelle VAN LEENE (56 Belgium), Geert DE JAEGER (56 Belgium), Steffen VANNESTE (56 Belgium) and Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution).
Edition Molecular Plant, Oxford, Oxford University Press, 2013, 1674-2052.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 6.605
RIV identification code RIV/00216224:14740/13:00072087
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/mp/sst044
UT WoS 000327541200010
Keywords in English retromer; VPS35; VPS29; prevacuolar compartment (PVC); vacuolar trafficking; Arabidopsis thaliana
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 13. 2. 2014 20:51.
Abstract
Arabidopsis mutants defective in subunits of the retromer complex VPS35A and VPS29 show comparable defects in subcellular trafficking and ectopic intracellular accumulation of membrane proteins. These retromer components mediate together the function of the prevacuolar compartment (PVC) and trafficking to the lytic vacuole.Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 (pat3) mutant that we identified by an epifluorescence-based forward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1GFP. While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
EE2.3.30.0009, research and development projectName: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
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