Overexpression of the Auxin Binding PROTEIN1 Modulates
PIN-Dependent Auxin Transport in Tobacco Cells

J 2013

Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

COVANOVA, Milada, Michael SAUER, Jan RYCHTAR, Jiří FRIML, Jan PETRASEK et. al.

Základní údaje

Originální název

Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

Autoři

COVANOVA, Milada (203 Česká republika), Michael SAUER (56 Belgie), Jan RYCHTAR (840 Spojené státy), Jiří FRIML (203 Česká republika, garant, domácí), Jan PETRASEK (203 Česká republika) a Eva ZAŽÍMALOVÁ (203 Česká republika)

Vydání

Plos One, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2013, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.534

Kód RIV

RIV/00216224:14740/13:00072089

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1371/journal.pone.0070050

UT WoS

000325211000181

Klíčová slova anglicky

ZEA-MAYS-L; PLANT HORMONE AUXIN; MEMBRANE H+-ATPASE; PLASMA-MEMBRANE; COLEOPTILE MEMBRANES; MOLECULAR-CLONING; POLAR TRANSPORT; ARABIDOPSIS; EFFLUX; DIVISION

Štítky

ok, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 2. 2014 21:45, Olga Křížová

Anotace

V originále

Background: Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive. Methodology/Principal Findings: Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations. Conclusions/Significance: The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.

Citovat

COVANOVA, Milada, Michael SAUER, Jan RYCHTAR, Jiří FRIML, Jan PETRASEK a Eva ZAŽÍMALOVÁ. Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells. Plos One. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, roč. 8, č. 7, s. "nestránkováno", 11 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0070050.

@article{1165391,
   author = {Covanova, Milada and Sauer, Michael and Rychtar, Jan and Friml, Jiří and Petrasek, Jan and Zažímalová, Eva},
   article_location = {SAN FRANCISCO},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0070050},
   keywords = {ZEA-MAYS-L; PLANT HORMONE AUXIN; MEMBRANE H+-ATPASE; PLASMA-MEMBRANE; COLEOPTILE MEMBRANES; MOLECULAR-CLONING; POLAR TRANSPORT; ARABIDOPSIS; EFFLUX; DIVISION},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos One},
   title = {Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1165391
AU  - Covanova, Milada - Sauer, Michael - Rychtar, Jan - Friml, Jiří - Petrasek, Jan - Zažímalová, Eva
PY  - 2013
TI  - Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells
JF  - Plos One
VL  - 8
IS  - 7
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - ZEA-MAYS-L
KW  - PLANT HORMONE AUXIN
KW  - MEMBRANE H+-ATPASE
KW  - PLASMA-MEMBRANE
KW  - COLEOPTILE MEMBRANES
KW  - MOLECULAR-CLONING
KW  - POLAR TRANSPORT
KW  - ARABIDOPSIS
KW  - EFFLUX
KW  - DIVISION
N2  - Background: Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive. Methodology/Principal Findings: Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations. Conclusions/Significance: The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.
ER  -

COVANOVA, Milada, Michael SAUER, Jan RYCHTAR, Jiří FRIML, Jan PETRASEK a Eva ZAŽÍMALOVÁ. Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells. \textit{Plos One}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, roč.~8, č.~7, s.~''nestránkováno'', 11 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0070050.

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