Overexpression of the Auxin Binding PROTEIN1 Modulates
PIN-Dependent Auxin Transport in Tobacco Cells

J 2013

Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

COVANOVA, Milada, Michael SAUER, Jan RYCHTAR, Jiří FRIML, Jan PETRASEK et. al.

Basic information

Original name

Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells

Authors

COVANOVA, Milada (203 Czech Republic), Michael SAUER (56 Belgium), Jan RYCHTAR (840 United States of America), Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution), Jan PETRASEK (203 Czech Republic) and Eva ZAŽÍMALOVÁ (203 Czech Republic)

Edition

Plos One, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2013, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.534

RIV identification code

RIV/00216224:14740/13:00072089

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0070050

UT WoS

000325211000181

Keywords in English

ZEA-MAYS-L; PLANT HORMONE AUXIN; MEMBRANE H+-ATPASE; PLASMA-MEMBRANE; COLEOPTILE MEMBRANES; MOLECULAR-CLONING; POLAR TRANSPORT; ARABIDOPSIS; EFFLUX; DIVISION

Abstract

V originále

Background: Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive. Methodology/Principal Findings: Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations. Conclusions/Significance: The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.

Citovat

COVANOVA, Milada, Michael SAUER, Jan RYCHTAR, Jiří FRIML, Jan PETRASEK and Eva ZAŽÍMALOVÁ. Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells. Plos One. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, vol. 8, No 7, p. "nestránkováno", 11 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0070050.

@article{1165391,
   author = {Covanova, Milada and Sauer, Michael and Rychtar, Jan and Friml, Jiří and Petrasek, Jan and Zažímalová, Eva},
   article_location = {SAN FRANCISCO},
   article_number = {7},
   doi = {http://dx.doi.org/10.1371/journal.pone.0070050},
   keywords = {ZEA-MAYS-L; PLANT HORMONE AUXIN; MEMBRANE H+-ATPASE; PLASMA-MEMBRANE; COLEOPTILE MEMBRANES; MOLECULAR-CLONING; POLAR TRANSPORT; ARABIDOPSIS; EFFLUX; DIVISION},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos One},
   title = {Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells},
   volume = {8},
   year = {2013}
}

TY  - JOUR
ID  - 1165391
AU  - Covanova, Milada - Sauer, Michael - Rychtar, Jan - Friml, Jiří - Petrasek, Jan - Zažímalová, Eva
PY  - 2013
TI  - Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells
JF  - Plos One
VL  - 8
IS  - 7
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - ZEA-MAYS-L
KW  - PLANT HORMONE AUXIN
KW  - MEMBRANE H+-ATPASE
KW  - PLASMA-MEMBRANE
KW  - COLEOPTILE MEMBRANES
KW  - MOLECULAR-CLONING
KW  - POLAR TRANSPORT
KW  - ARABIDOPSIS
KW  - EFFLUX
KW  - DIVISION
N2  - Background: Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive. Methodology/Principal Findings: Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations. Conclusions/Significance: The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients.
ER  -

COVANOVA, Milada, Michael SAUER, Jan RYCHTAR, Jiří FRIML, Jan PETRASEK and Eva ZAŽÍMALOVÁ. Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells. \textit{Plos One}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2013, vol.~8, No~7, p.~''nestránkováno'', 11 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0070050.

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