BARBEZ, Elke, Martina LANKOVA, Marketa PAREZOVA, Alexis MAIZEL, Eva ZAŽÍMALOVÁ, Jan PETRASEK, Jiří FRIML and Jurgen KLEINE-VEHN. Single-cell-based system to monitor carrier driven cellular auxin homeostasis. BMC Plant Biology. London: BioMed Central Ltd., 2013, vol. 13, February, p. "nestránkováno", 13 pp. ISSN 1471-2229. doi:10.1186/1471-2229-13-20.
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Basic information
Original name Single-cell-based system to monitor carrier driven cellular auxin homeostasis
Authors BARBEZ, Elke (56 Belgium), Martina LANKOVA (203 Czech Republic), Marketa PAREZOVA (203 Czech Republic), Alexis MAIZEL (276 Germany), Eva ZAŽÍMALOVÁ (203 Czech Republic), Jan PETRASEK (203 Czech Republic), Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution) and Jurgen KLEINE-VEHN (56 Belgium).
Edition BMC Plant Biology, London, BioMed Central Ltd. 2013, 1471-2229.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.942
RIV identification code RIV/00216224:14740/13:00072091
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1186/1471-2229-13-20
UT WoS 000315715700001
Keywords in English Auxin homeostasis; DR5; Auxin carrier; Auxin transport
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 10/12/2019 11:44.
Abstract
Background: Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance of auxin transporters, a simple medium-to-high throughput approach to assess carrier activities is still missing. Here we show that carrier driven depletion of cellular auxin correlates with reduced nuclear auxin signaling in tobacco Bright Yellow-2 (BY-2) cell cultures. Results: We developed an easy to use transient single-cell-based system to detect carrier activity. We use the relative changes in signaling output of the auxin responsive promoter element DR5 to indirectly visualize auxin carrier activity. The feasibility of the transient approach was demonstrated by pharmacological and genetic interference with auxin signaling and transport. As a proof of concept, we provide visual evidence that the prominent auxin transport proteins PIN-FORMED (PIN) 2 and PIN5 regulate cellular auxin homeostasis at the plasma membrane and endoplasmic reticulum (ER), respectively. Our data suggest that PIN2 and PIN5 have different sensitivities to the auxin transport inhibitor 1-naphthylphthalamic acid (NPA). Also the putative PIN-LIKES (PILS) auxin carrier activity at the ER is insensitive to NPA in our system, indicating that NPA blocks intercellular, but not intracellular auxin transport. Conclusions: This single-cell-based system is a useful tool by which the activity of putative auxin carriers, such as PINs, PILS and WALLS ARE THIN1 (WAT1), can be indirectly visualized in a medium-to-high throughput manner. Moreover, our single cell system might be useful to investigate also other hormonal signaling pathways, such as cytokinin.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
EE2.3.20.0043, research and development projectName: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů
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