Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin
Exporter ABCB1 on the Root Plasma Membrane

J 2013

Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane

WANG, Bangjun, Aurelien BAILLY, Marta ZWIEWKA, Sina HENRICHS, Elisa AZZARELLO et. al.

Basic information

Original name

Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane

Authors

WANG, Bangjun (756 Switzerland), Aurelien BAILLY (756 Switzerland), Marta ZWIEWKA (616 Poland), Sina HENRICHS (756 Switzerland), Elisa AZZARELLO (380 Italy), Stefano MANCUSO (56 Belgium), Masayoshi MAESHIMA (392 Japan), Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution), Alexander SCHULZ (208 Denmark) and Markus GEISLER (756 Switzerland)

Edition

Plant Cell, ROCKVILLE, AMER SOC PLANT BIOLOGISTS, 2013, 1040-4651

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.575

RIV identification code

RIV/00216224:14740/13:00072092

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1105/tpc.112.105999

UT WoS

000315572400017

Keywords in English

BINDING CASSETTE TRANSPORTER; PLANT DEVELOPMENT; P-GLYCOPROTEINS; LATERAL ROOT; EFFLUX; IMMUNOPHILIN; RESISTANCE; CELLS; PIN; LOCALIZATION

Abstract

V originále

Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.

Citovat

WANG, Bangjun, Aurelien BAILLY, Marta ZWIEWKA, Sina HENRICHS, Elisa AZZARELLO, Stefano MANCUSO, Masayoshi MAESHIMA, Jiří FRIML, Alexander SCHULZ and Markus GEISLER. Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2013, vol. 25, No 1, p. 202-214. ISSN 1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.105999.

@article{1165395,
   author = {Wang, Bangjun and Bailly, Aurelien and Zwiewka, Marta and Henrichs, Sina and Azzarello, Elisa and Mancuso, Stefano and Maeshima, Masayoshi and Friml, Jiří and Schulz, Alexander and Geisler, Markus},
   article_location = {ROCKVILLE},
   article_number = {1},
   doi = {http://dx.doi.org/10.1105/tpc.112.105999},
   keywords = {BINDING CASSETTE TRANSPORTER; PLANT DEVELOPMENT; P-GLYCOPROTEINS; LATERAL ROOT; EFFLUX; IMMUNOPHILIN; RESISTANCE; CELLS; PIN; LOCALIZATION},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane},
   url = {http://www.plantcell.org/content/25/1/202},
   volume = {25},
   year = {2013}
}

TY  - JOUR
ID  - 1165395
AU  - Wang, Bangjun - Bailly, Aurelien - Zwiewka, Marta - Henrichs, Sina - Azzarello, Elisa - Mancuso, Stefano - Maeshima, Masayoshi - Friml, Jiří - Schulz, Alexander - Geisler, Markus
PY  - 2013
TI  - Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane
JF  - Plant Cell
VL  - 25
IS  - 1
SP  - 202-214
EP  - 202-214
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - BINDING CASSETTE TRANSPORTER
KW  - PLANT DEVELOPMENT
KW  - P-GLYCOPROTEINS
KW  - LATERAL ROOT
KW  - EFFLUX
KW  - IMMUNOPHILIN
KW  - RESISTANCE
KW  - CELLS
KW  - PIN
KW  - LOCALIZATION
UR  - http://www.plantcell.org/content/25/1/202
L2  - http://www.plantcell.org/content/25/1/202
N2  - Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.
ER  -

WANG, Bangjun, Aurelien BAILLY, Marta ZWIEWKA, Sina HENRICHS, Elisa AZZARELLO, Stefano MANCUSO, Masayoshi MAESHIMA, Jiří FRIML, Alexander SCHULZ and Markus GEISLER. Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane. \textit{Plant Cell}. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2013, vol.~25, No~1, p.~202-214. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.105999.

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