WANG, Bangjun, Aurelien BAILLY, Marta ZWIEWKA, Sina HENRICHS, Elisa AZZARELLO, Stefano MANCUSO, Masayoshi MAESHIMA, Jiří FRIML, Alexander SCHULZ and Markus GEISLER. Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane. Plant Cell. ROCKVILLE: AMER SOC PLANT BIOLOGISTS, 2013, vol. 25, No 1, p. 202-214. ISSN 1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.105999.
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Basic information
Original name Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter ABCB1 on the Root Plasma Membrane
Authors WANG, Bangjun (756 Switzerland), Aurelien BAILLY (756 Switzerland), Marta ZWIEWKA (616 Poland), Sina HENRICHS (756 Switzerland), Elisa AZZARELLO (380 Italy), Stefano MANCUSO (56 Belgium), Masayoshi MAESHIMA (392 Japan), Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution), Alexander SCHULZ (208 Denmark) and Markus GEISLER (756 Switzerland).
Edition Plant Cell, ROCKVILLE, AMER SOC PLANT BIOLOGISTS, 2013, 1040-4651.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 9.575
RIV identification code RIV/00216224:14740/13:00072092
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1105/tpc.112.105999
UT WoS 000315572400017
Keywords in English BINDING CASSETTE TRANSPORTER; PLANT DEVELOPMENT; P-GLYCOPROTEINS; LATERAL ROOT; EFFLUX; IMMUNOPHILIN; RESISTANCE; CELLS; PIN; LOCALIZATION
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 10/4/2014 09:25.
Abstract
Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.
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