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@article{1166319,
author = {Šedo, Ondrej and Vávrová, Andrea and Medková, Marta and Tvrzová, Ludmila and Zdráhal, Zbyněk},
article_location = {Hoboken, USA},
article_number = {24},
doi = {http://dx.doi.org/10.1002/rcm.6741},
keywords = {DESORPTION-IONIZATION-TIME; MALDI-TOF-MS; LACTIC-ACID BACTERIA; STAPHYLOCOCCUS-AUREUS; RAPID IDENTIFICATION; MICROORGANISM IDENTIFICATION; WHOLE CELLS; CLASSIFICATION; DISCRIMINATION; CULTURE},
language = {eng},
issn = {0951-4198},
journal = {Rapid Communications in Mass Spectrometry},
title = {The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling},
url = {http://onlinelibrary.wiley.com/doi/10.1002/rcm.6741/abstract;jsessionid=80166C0ED5AC8BAA86C882BA5C778425.f03t03},
volume = {27},
year = {2013}
}
TY - JOUR
ID - 1166319
AU - Šedo, Ondrej - Vávrová, Andrea - Medková, Marta - Tvrzová, Ludmila - Zdráhal, Zbyněk
PY - 2013
TI - The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling
JF - Rapid Communications in Mass Spectrometry
VL - 27
IS - 24
SP - 2729-2736
EP - 2729-2736
PB - WILEY-BLACKWELL
SN - 09514198
KW - DESORPTION-IONIZATION-TIME
KW - MALDI-TOF-MS
KW - LACTIC-ACID BACTERIA
KW - STAPHYLOCOCCUS-AUREUS
KW - RAPID IDENTIFICATION
KW - MICROORGANISM IDENTIFICATION
KW - WHOLE CELLS
KW - CLASSIFICATION
KW - DISCRIMINATION
KW - CULTURE
UR - http://onlinelibrary.wiley.com/doi/10.1002/rcm.6741/abstract;jsessionid=80166C0ED5AC8BAA86C882BA5C778425.f03t03
L2 - http://onlinelibrary.wiley.com/doi/10.1002/rcm.6741/abstract;jsessionid=80166C0ED5AC8BAA86C882BA5C778425.f03t03
N2 - For the purpose of this study, we used an extraction protocol recommended for clinical laboratories for MALDI-TOF MS profiling of bacteria. Seventeen closely related strains of the Lactobacillus acidophilus group were cultivated under various growth conditions (growth medium, time, and temperature) and analyzed. Out of a total of 327 samples, 80 % were correctly assigned to the species level and 13 % only to the genus level. When using data obtained from strains cultured for lengthy periods (7 days), the identification success rate was reduced due to poor signal quality, whereas with shorter cultivation times there was no influence of growth conditions on the assignment of particular strains to their corresponding species. However, variations in certain cultivation parameters were found to influence identification and differentiation of most of the examined strains. Strain discrimination was frequently found to be dependent on the selection of culture conditions. MALDI-TOF MS data treatment (strain-specific peak detection, BioTyper scoring, subtyping, or cluster analysis) also contributed to the discriminatory power of the method.
ER -
ŠEDO, Ondrej, Andrea VÁVROVÁ, Marta MEDKOVÁ, Ludmila TVRZOVÁ a Zbyněk ZDRÁHAL. The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling. \textit{Rapid Communications in Mass Spectrometry}. Hoboken, USA: WILEY-BLACKWELL, 2013, roč.~27, č.~24, s.~2729-2736. ISSN~0951-4198. doi:10.1002/rcm.6741.
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