ŠEDO, Ondrej, Andrea VÁVROVÁ, Marta MEDKOVÁ, Ludmila TVRZOVÁ and Zbyněk ZDRÁHAL. The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling. Rapid Communications in Mass Spectrometry. Hoboken, USA: WILEY-BLACKWELL, 2013, vol. 27, No 24, p. 2729-2736. ISSN 0951-4198. doi:10.1002/rcm.6741.
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Basic information
Original name The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling
Authors ŠEDO, Ondrej (203 Czech Republic, belonging to the institution), Andrea VÁVROVÁ (203 Czech Republic, belonging to the institution), Marta MEDKOVÁ (203 Czech Republic, belonging to the institution), Ludmila TVRZOVÁ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution).
Edition Rapid Communications in Mass Spectrometry, Hoboken, USA, WILEY-BLACKWELL, 2013, 0951-4198.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.642
RIV identification code RIV/00216224:14740/13:00066925
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1002/rcm.6741
UT WoS 000330107600001
Keywords in English DESORPTION-IONIZATION-TIME; MALDI-TOF-MS; LACTIC-ACID BACTERIA; STAPHYLOCOCCUS-AUREUS; RAPID IDENTIFICATION; MICROORGANISM IDENTIFICATION; WHOLE CELLS; CLASSIFICATION; DISCRIMINATION; CULTURE
Tags podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 5. 4. 2014 07:37.
Abstract
For the purpose of this study, we used an extraction protocol recommended for clinical laboratories for MALDI-TOF MS profiling of bacteria. Seventeen closely related strains of the Lactobacillus acidophilus group were cultivated under various growth conditions (growth medium, time, and temperature) and analyzed. Out of a total of 327 samples, 80 % were correctly assigned to the species level and 13 % only to the genus level. When using data obtained from strains cultured for lengthy periods (7 days), the identification success rate was reduced due to poor signal quality, whereas with shorter cultivation times there was no influence of growth conditions on the assignment of particular strains to their corresponding species. However, variations in certain cultivation parameters were found to influence identification and differentiation of most of the examined strains. Strain discrimination was frequently found to be dependent on the selection of culture conditions. MALDI-TOF MS data treatment (strain-specific peak detection, BioTyper scoring, subtyping, or cluster analysis) also contributed to the discriminatory power of the method.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GA13-26693S, research and development projectName: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.
Investor: Czech Science Foundation
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