The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling

ŠEDO, Ondrej, Andrea VÁVROVÁ, Marta MEDKOVÁ, Ludmila TVRZOVÁ and Zbyněk ZDRÁHAL. The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling. Rapid Communications in Mass Spectrometry. Hoboken, USA: WILEY-BLACKWELL, 2013, vol. 27, No 24, p. 2729-2736. ISSN 0951-4198. doi:10.1002/rcm.6741.

Basic information

• Original name: The influence of growth conditions on strain differentiation within the Lactobacillus acidophilus group using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling
• Authors: ŠEDO, Ondrej (203 Czech Republic, belonging to the institution), Andrea VÁVROVÁ (203 Czech Republic, belonging to the institution), Marta MEDKOVÁ (203 Czech Republic, belonging to the institution), Ludmila TVRZOVÁ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Rapid Communications in Mass Spectrometry, Hoboken, USA, WILEY-BLACKWELL, 2013, 0951-4198.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.642
• RIV identification code: RIV/00216224:14740/13:00066925
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/rcm.6741
• UT WoS: 000330107600001
• Keywords in English: DESORPTION-IONIZATION-TIME; MALDI-TOF-MS; LACTIC-ACID BACTERIA; STAPHYLOCOCCUS-AUREUS; RAPID IDENTIFICATION; MICROORGANISM IDENTIFICATION; WHOLE CELLS; CLASSIFICATION; DISCRIMINATION; CULTURE
• Tags: podil, rivok
• Tags: International impact, Reviewed

Abstract

For the purpose of this study, we used an extraction protocol recommended for clinical laboratories for MALDI-TOF MS profiling of bacteria. Seventeen closely related strains of the Lactobacillus acidophilus group were cultivated under various growth conditions (growth medium, time, and temperature) and analyzed. Out of a total of 327 samples, 80 % were correctly assigned to the species level and 13 % only to the genus level. When using data obtained from strains cultured for lengthy periods (7 days), the identification success rate was reduced due to poor signal quality, whereas with shorter cultivation times there was no influence of growth conditions on the assignment of particular strains to their corresponding species. However, variations in certain cultivation parameters were found to influence identification and differentiation of most of the examined strains. Strain discrimination was frequently found to be dependent on the selection of culture conditions. MALDI-TOF MS data treatment (strain-specific peak detection, BioTyper scoring, subtyping, or cluster analysis) also contributed to the discriminatory power of the method.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GA13-26693S, research and development project: Name: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.

Investor: Czech Science Foundation