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@article{1167373,
author = {Šobrová, Pavlína and Ryvolová, Markéta and Pekařík, Vladimír and Hubálek, Jaromír and Adam, Vojtěch and Kizek, René},
article_location = {Belgrade},
article_number = {12},
keywords = {Quantum Dot; Prion Proteins; In Vivo Imaging; Electrochemistry; Differential Pulse Voltammetry},
language = {eng},
issn = {1452-3981},
journal = {International Journal of Electrochemical Science},
title = {Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots},
url = {http://www.electrochemsci.org/papers/vol8/81212466.pdf},
volume = {8},
year = {2013}
}
TY - JOUR
ID - 1167373
AU - Šobrová, Pavlína - Ryvolová, Markéta - Pekařík, Vladimír - Hubálek, Jaromír - Adam, Vojtěch - Kizek, René
PY - 2013
TI - Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots
JF - International Journal of Electrochemical Science
VL - 8
IS - 12
SP - 12466-12475
EP - 12466-12475
PB - ESG
SN - 14523981
KW - Quantum Dot
KW - Prion Proteins
KW - In Vivo Imaging
KW - Electrochemistry
KW - Differential Pulse Voltammetry
UR - http://www.electrochemsci.org/papers/vol8/81212466.pdf
L2 - http://www.electrochemsci.org/papers/vol8/81212466.pdf
N2 - The prion protein (PrP) is involved in neurodegeneration via its conversion from the normal cellular form, PrPC, to the infectious form, PrPSc, which is the causative agent of the transmissible spongiform encephalopathies (TSEs) including Creutzfeldt-Jakob disease (CJD). In spite of great effort in this field, diagnostics of prion protein caused diseases represents a sort of challenge. In this study, we aimed our attention on studying of prion protein interaction with CdTe quantum dots (QDs) by voltammetry as a new and extremely sensitive tool for sensing of these proteins. Primarily, we characterized fluorescent and electrochemical properties of QDs. Further, electrochemical study of their interactions was carried out to find the most suitable conditions for sensitive detection of prion proteins. Detection limit (3 S/N) was estimated as 1 fg in 5 mu l. This makes labeling of proteins with QDs of great importance due to easy applicability and possibility to use in miniaturized devices, which can be used in situ. Based on our results it can be concluded that QDs-prion protein complex is stable and can be quantified in extremely low amounts. This should open new possibilities how to determine the presence of these proteins on surgical equipment and other types of materials, which could be contagious.
ER -
ŠOBROVÁ, Pavlína, Markéta RYVOLOVÁ, Vladimír PEKAŘÍK, Jaromír HUBÁLEK, Vojtěch ADAM and René KIZEK. Femtogram Electrochemical Sensing of Prion Proteins Using Quantum Dots. \textit{International Journal of Electrochemical Science}. Belgrade: ESG, 2013, vol.~8, No~12, p.~12466-12475. ISSN~1452-3981.
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