Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth

BAILLY, Auréllien, Bangjun WANG, Marta ZWIEWKA, Stephan POLLMANN, Daniel SCHENCK, Hartwig LÜTHEN, Alexander SCHULTZ, Jiří FRIML and Markus GEISLER. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. The Plant Journal. USA: Blackwell Science, vol. 77, No 1, p. 108-118. ISSN 0960-7412. doi:10.1111/tpj.12369. 2014.

Basic information

• Original name: Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth
• Authors: BAILLY, Auréllien (756 Switzerland, guarantor), Bangjun WANG (756 Switzerland), Marta ZWIEWKA (616 Poland, belonging to the institution), Stephan POLLMANN (276 Germany), Daniel SCHENCK (276 Germany), Hartwig LÜTHEN (276 Germany), Alexander SCHULTZ (276 Germany), Jiří FRIML (203 Czech Republic, belonging to the institution) and Markus GEISLER (756 Switzerland).
• Edition: The Plant Journal, USA, Blackwell Science, 2014, 0960-7412.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.972
• RIV identification code: RIV/00216224:14740/14:00073518
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1111/tpj.12369
• UT WoS: 000328661300009
• Keywords in English: polar auxin transport; ABCB; P-glycoprotein; TWISTED DWARF1; cell elongation
• Tags: kontrola MP, rivok
• Tags: International impact, Reviewed

Abstract

Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor-glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct ) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct . As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• EE2.3.20.0043, research and development project: Name: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů
• EE2.3.30.0009, research and development project: Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
• GA13-40637S, research and development project: Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation