Expression of TWISTED DWARF1 lacking its in-plane membrane
anchor leads to increased cell elongation and hypermorphic
growth

J 2014

Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth

BAILLY, Auréllien, Bangjun WANG, Marta ZWIEWKA, Stephan POLLMANN, Daniel SCHENCK et. al.

Basic information

Original name

Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth

Authors

BAILLY, Auréllien (756 Switzerland, guarantor), Bangjun WANG (756 Switzerland), Marta ZWIEWKA (616 Poland, belonging to the institution), Stephan POLLMANN (276 Germany), Daniel SCHENCK (276 Germany), Hartwig LÜTHEN (276 Germany), Alexander SCHULTZ (276 Germany), Jiří FRIML (203 Czech Republic, belonging to the institution) and Markus GEISLER (756 Switzerland)

Edition

The Plant Journal, USA, Blackwell Science, 2014, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.972

RIV identification code

RIV/00216224:14740/14:00073518

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/tpj.12369

UT WoS

000328661300009

Keywords in English

polar auxin transport; ABCB; P-glycoprotein; TWISTED DWARF1; cell elongation

Abstract

V originále

Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor-glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct ) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct . As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0043, research and development project

Name: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

Citovat

BAILLY, Auréllien, Bangjun WANG, Marta ZWIEWKA, Stephan POLLMANN, Daniel SCHENCK, Hartwig LÜTHEN, Alexander SCHULTZ, Jiří FRIML and Markus GEISLER. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. The Plant Journal. USA: Blackwell Science, 2014, vol. 77, No 1, p. 108-118. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12369.

@article{1168182,
   author = {Bailly, Auréllien and Wang, Bangjun and Zwiewka, Marta and Pollmann, Stephan and Schenck, Daniel and Lüthen, Hartwig and Schultz, Alexander and Friml, Jiří and Geisler, Markus},
   article_location = {USA},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/tpj.12369},
   keywords = {polar auxin transport; ABCB; P-glycoprotein; TWISTED DWARF1; cell elongation},
   language = {eng},
   issn = {0960-7412},
   journal = {The Plant Journal},
   title = {Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.12369/abstract},
   volume = {77},
   year = {2014}
}

TY  - JOUR
ID  - 1168182
AU  - Bailly, Auréllien - Wang, Bangjun - Zwiewka, Marta - Pollmann, Stephan - Schenck, Daniel - Lüthen, Hartwig - Schultz, Alexander - Friml, Jiří - Geisler, Markus
PY  - 2014
TI  - Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth
JF  - The Plant Journal
VL  - 77
IS  - 1
SP  - 108-118
EP  - 108-118
PB  - Blackwell Science
SN  - 09607412
KW  - polar auxin transport
KW  - ABCB
KW  - P-glycoprotein
KW  - TWISTED DWARF1
KW  - cell elongation
UR  - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12369/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12369/abstract
N2  - Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor-glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct ) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct . As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation.
ER  -

BAILLY, Auréllien, Bangjun WANG, Marta ZWIEWKA, Stephan POLLMANN, Daniel SCHENCK, Hartwig LÜTHEN, Alexander SCHULTZ, Jiří FRIML and Markus GEISLER. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. \textit{The Plant Journal}. USA: Blackwell Science, 2014, vol.~77, No~1, p.~108-118. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.12369.

Detailed Information on Publication Record