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@article{1169449,
author = {Adamovský, Ondřej and Palíková, Miroslava and Ondrackova, Petra and Zikova, Andrea and Kopp, Radovan and Mares, Jan and Pikula, Jiri and Paskerová, Hana and Kohoutek, Jiří and Bláha, Luděk},
article_location = {Sweden, Stockholm},
article_number = {2},
keywords = {Cyanobacteria; laboratory rats; experimental exposure; oxidative stress; biochemistry; histopathology},
language = {eng},
issn = {0172-780X},
journal = {Neuroendocrinology Letters},
title = {Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass},
volume = {34},
year = {2013}
}
TY - JOUR
ID - 1169449
AU - Adamovský, Ondřej - Palíková, Miroslava - Ondrackova, Petra - Zikova, Andrea - Kopp, Radovan - Mares, Jan - Pikula, Jiri - Paskerová, Hana - Kohoutek, Jiří - Bláha, Luděk
PY - 2013
TI - Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass
JF - Neuroendocrinology Letters
VL - 34
IS - 2
SP - 11-20
EP - 11-20
PB - Maghira and Maas Publications
SN - 0172780X
KW - Cyanobacteria
KW - laboratory rats
KW - experimental exposure
KW - oxidative stress
KW - biochemistry
KW - histopathology
N2 - OBJECTIVES: Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of pure microcystins and cyanobacterial biomass on laboratory rats (Wistar albino rats, males, 30 days old) under different exposure scenarios. METHODS: The rats were fed diets containing fish meat with microcystins in various concentrations and forms (cyanobacterial biomass and isolated microcystins) for 28 days. RESULTS: Although considerable amounts of microcystins (MCs) were administered to the rats, all levels of MCs in the liver were close to the detection limit (3-5 ng/g fresh weight) using liquid chromatography - tandem mass spectrometry. Only rats exposed to cyanobacterial biomass had clearly higher hepatic and splenic somatic indexes while markers of oxidative stress (glutathione-S-transferase, glutathione reductase, lipid peroxidatio) were significantly increased in the group exposed to the high dose of MCs. Most of the analysed biochemical parameters did not show clear differences among groups. Levels of bilirubin and lipases were significantly increased only after exposure to cyanobacterial biomass and MCs, respectively. Considering microscopic findings in the liver, kidney, thymus, spleen and brain, histopathology was dominated by alterations in the hepatic parenchyma and renal cortical tubular system. CONCLUSIONS: The present study demonstrates that oral exposure to MCs and cyanobacterial biomass may induce biochemical and detoxification responses associated with damage to liver and kidneys and in the laboratory rat.
ER -
ADAMOVSKÝ, Ondřej, Miroslava PALÍKOVÁ, Petra ONDRACKOVA, Andrea ZIKOVA, Radovan KOPP, Jan MARES, Jiri PIKULA, Hana PASKEROVÁ, Jiří KOHOUTEK and Luděk BLÁHA. Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass. \textit{Neuroendocrinology Letters}. Sweden, Stockholm: Maghira and Maas Publications, 2013, vol.~34, No~2, p.~11-20. ISSN~0172-780X.
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