Biochemical and histopathological responses of Wistar rats to
oral intake of microcystins and cyanobacterial biomass

J 2013

Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass

ADAMOVSKÝ, Ondřej, Miroslava PALÍKOVÁ, Petra ONDRACKOVA, Andrea ZIKOVA, Radovan KOPP et. al.

Basic information

Original name

Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass

Authors

ADAMOVSKÝ, Ondřej (203 Czech Republic, guarantor, belonging to the institution), Miroslava PALÍKOVÁ (203 Czech Republic), Petra ONDRACKOVA (203 Czech Republic), Andrea ZIKOVA (276 Germany), Radovan KOPP (203 Czech Republic), Jan MARES (203 Czech Republic), Jiri PIKULA (203 Czech Republic), Hana PASKEROVÁ (203 Czech Republic), Jiří KOHOUTEK (203 Czech Republic, belonging to the institution) and Luděk BLÁHA (203 Czech Republic, belonging to the institution)

Edition

Neuroendocrinology Letters, Sweden, Stockholm, Maghira and Maas Publications, 2013, 0172-780X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Sweden

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.935

RIV identification code

RIV/00216224:14310/13:00065693

Organization unit

Faculty of Science

UT WoS

000330901000002

Keywords in English

Cyanobacteria; laboratory rats; experimental exposure; oxidative stress; biochemistry; histopathology

Abstract

V originále

OBJECTIVES: Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of pure microcystins and cyanobacterial biomass on laboratory rats (Wistar albino rats, males, 30 days old) under different exposure scenarios. METHODS: The rats were fed diets containing fish meat with microcystins in various concentrations and forms (cyanobacterial biomass and isolated microcystins) for 28 days. RESULTS: Although considerable amounts of microcystins (MCs) were administered to the rats, all levels of MCs in the liver were close to the detection limit (3-5 ng/g fresh weight) using liquid chromatography - tandem mass spectrometry. Only rats exposed to cyanobacterial biomass had clearly higher hepatic and splenic somatic indexes while markers of oxidative stress (glutathione-S-transferase, glutathione reductase, lipid peroxidatio) were significantly increased in the group exposed to the high dose of MCs. Most of the analysed biochemical parameters did not show clear differences among groups. Levels of bilirubin and lipases were significantly increased only after exposure to cyanobacterial biomass and MCs, respectively. Considering microscopic findings in the liver, kidney, thymus, spleen and brain, histopathology was dominated by alterations in the hepatic parenchyma and renal cortical tubular system. CONCLUSIONS: The present study demonstrates that oral exposure to MCs and cyanobacterial biomass may induce biochemical and detoxification responses associated with damage to liver and kidneys and in the laboratory rat.

Links

ED0001/01/01, research and development project

Name: CETOCOEN

QH71015, research and development project

Name: Minimalizace rizik výskytu metabolitů sinic v technologických procesech rybářského sektoru.

Investor: Ministry of Agriculture of the CR, Risk Minimization of Cyanobacterial Metabolite Occurrence in Technologic Processes of Fishery Sector

Citovat

ADAMOVSKÝ, Ondřej, Miroslava PALÍKOVÁ, Petra ONDRACKOVA, Andrea ZIKOVA, Radovan KOPP, Jan MARES, Jiri PIKULA, Hana PASKEROVÁ, Jiří KOHOUTEK and Luděk BLÁHA. Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass. Neuroendocrinology Letters. Sweden, Stockholm: Maghira and Maas Publications, 2013, vol. 34, No 2, p. 11-20. ISSN 0172-780X.

@article{1169449,
   author = {Adamovský, Ondřej and Palíková, Miroslava and Ondrackova, Petra and Zikova, Andrea and Kopp, Radovan and Mares, Jan and Pikula, Jiri and Paskerová, Hana and Kohoutek, Jiří and Bláha, Luděk},
   article_location = {Sweden, Stockholm},
   article_number = {2},
   keywords = {Cyanobacteria; laboratory rats; experimental exposure; oxidative stress; biochemistry; histopathology},
   language = {eng},
   issn = {0172-780X},
   journal = {Neuroendocrinology Letters},
   title = {Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass},
   volume = {34},
   year = {2013}
}

TY  - JOUR
ID  - 1169449
AU  - Adamovský, Ondřej - Palíková, Miroslava - Ondrackova, Petra - Zikova, Andrea - Kopp, Radovan - Mares, Jan - Pikula, Jiri - Paskerová, Hana - Kohoutek, Jiří - Bláha, Luděk
PY  - 2013
TI  - Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass
JF  - Neuroendocrinology Letters
VL  - 34
IS  - 2
SP  - 11-20
EP  - 11-20
PB  - Maghira and Maas Publications
SN  - 0172780X
KW  - Cyanobacteria
KW  - laboratory rats
KW  - experimental exposure
KW  - oxidative stress
KW  - biochemistry
KW  - histopathology
N2  - OBJECTIVES: Cyanobacteria are producers of potent and environmentally abundant microcystins, representing an emerging global health issue. In the present study, we investigated the impact of pure microcystins and cyanobacterial biomass on laboratory rats (Wistar albino rats, males, 30 days old) under different exposure scenarios. METHODS: The rats were fed diets containing fish meat with microcystins in various concentrations and forms (cyanobacterial biomass and isolated microcystins) for 28 days. RESULTS: Although considerable amounts of microcystins (MCs) were administered to the rats, all levels of MCs in the liver were close to the detection limit (3-5 ng/g fresh weight) using liquid chromatography - tandem mass spectrometry. Only rats exposed to cyanobacterial biomass had clearly higher hepatic and splenic somatic indexes while markers of oxidative stress (glutathione-S-transferase, glutathione reductase, lipid peroxidatio) were significantly increased in the group exposed to the high dose of MCs. Most of the analysed biochemical parameters did not show clear differences among groups. Levels of bilirubin and lipases were significantly increased only after exposure to cyanobacterial biomass and MCs, respectively. Considering microscopic findings in the liver, kidney, thymus, spleen and brain, histopathology was dominated by alterations in the hepatic parenchyma and renal cortical tubular system. CONCLUSIONS: The present study demonstrates that oral exposure to MCs and cyanobacterial biomass may induce biochemical and detoxification responses associated with damage to liver and kidneys and in the laboratory rat.
ER  -

ADAMOVSKÝ, Ondřej, Miroslava PALÍKOVÁ, Petra ONDRACKOVA, Andrea ZIKOVA, Radovan KOPP, Jan MARES, Jiri PIKULA, Hana PASKEROVÁ, Jiří KOHOUTEK and Luděk BLÁHA. Biochemical and histopathological responses of Wistar rats to oral intake of microcystins and cyanobacterial biomass. \textit{Neuroendocrinology Letters}. Sweden, Stockholm: Maghira and Maas Publications, 2013, vol.~34, No~2, p.~11-20. ISSN~0172-780X.

Detailed Information on Publication Record