KUBÁŇ, Petr, Eeva-Gerda KOBRIN and Kaljurand MIHKEL. POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS. In Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P. CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012, p. 128-133. ISBN 978-80-904959-1-3.
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Basic information
Original name POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS
Authors KUBÁŇ, Petr (203 Czech Republic, guarantor, belonging to the institution), Eeva-Gerda KOBRIN (233 Estonia) and Kaljurand MIHKEL (233 Estonia).
Edition Brno, CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, p. 128-133, 6 pp. 2012.
Publisher Ústav analytické chemie AV ČR, v. v. i
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14740/12:00081987
Organization unit Central European Institute of Technology
ISBN 978-80-904959-1-3
UT WoS 000329635000041
Keywords (in Czech) kondenzát vydechovaného vzduch; kapilární elektroforéza; kapilární elektroforéza; point of care diagnostika
Keywords in English Exhaled breath condensate; capillary electrophoresis; point of care diagnostics
Tags kontrola MP, rivok
Tags International impact
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 25/9/2015 10:21.
Abstract
Exhaled breath condensate has been analyzed for its ionic content by capillary electrophoresis with capacitively coupled contactless conductometric detection. Two devices for exhaled breath condensate collection were compared. These include a tube-in-tube cooled sampler and a simple zip-lock bag. The devices allow collection of small volumes (100-200 mu L) of exhaled breath condensate in less than 2 min. A method for quick (less than 3 min) simultaneous determination of inorganic cations, inorganic anions and organic anions from the samples using dual-opposite end injection principle with a short fused silica capillary (35 cm, 50 mu m i.d.) was optimized with final background electrolyte composition of 20 mM 2-(N-morpholino) ethanesulfonic acid, 20 mM L-histidine, 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 was used. It has been shown that changes of nitrite could be observed in acute inflammation of upper airways and in person with diagnosed mild chronic obstructive pulmonary disease, while the changes of other ions could also be observed.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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