POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF
CARE DIGNOSTICS

D 2012

POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS

KUBÁŇ, Petr, Eeva-Gerda KOBRIN and Kaljurand MIHKEL

Basic information

Original name

POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS

Authors

KUBÁŇ, Petr (203 Czech Republic, guarantor, belonging to the institution), Eeva-Gerda KOBRIN (233 Estonia) and Kaljurand MIHKEL (233 Estonia)

Edition

Brno, CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS, p. 128-133, 6 pp. 2012

Publisher

Ústav analytické chemie AV ČR, v. v. i

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14740/12:00081987

Organization unit

Central European Institute of Technology

ISBN

978-80-904959-1-3

UT WoS

000329635000041

Keywords (in Czech)

kondenzát vydechovaného vzduch; kapilární elektroforéza; kapilární elektroforéza; point of care diagnostika

Keywords in English

Exhaled breath condensate; capillary electrophoresis; point of care diagnostics

Abstract

V originále

Exhaled breath condensate has been analyzed for its ionic content by capillary electrophoresis with capacitively coupled contactless conductometric detection. Two devices for exhaled breath condensate collection were compared. These include a tube-in-tube cooled sampler and a simple zip-lock bag. The devices allow collection of small volumes (100-200 mu L) of exhaled breath condensate in less than 2 min. A method for quick (less than 3 min) simultaneous determination of inorganic cations, inorganic anions and organic anions from the samples using dual-opposite end injection principle with a short fused silica capillary (35 cm, 50 mu m i.d.) was optimized with final background electrolyte composition of 20 mM 2-(N-morpholino) ethanesulfonic acid, 20 mM L-histidine, 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 was used. It has been shown that changes of nitrite could be observed in acute inflammation of upper airways and in person with diagnosed mild chronic obstructive pulmonary disease, while the changes of other ions could also be observed.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

KUBÁŇ, Petr, Eeva-Gerda KOBRIN and Kaljurand MIHKEL. POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS. In Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P. CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012, p. 128-133. ISBN 978-80-904959-1-3.

@inproceedings{1169981,
   author = {Kubáň, Petr and Kobrin, EevaandGerda and Mihkel, Kaljurand},
   address = {Brno},
   booktitle = {CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS},
   editor = {Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P},
   keywords = {Exhaled breath condensate; capillary electrophoresis; point of care diagnostics},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-904959-1-3},
   pages = {128-133},
   publisher = {Ústav analytické chemie AV ČR, v. v. i},
   title = {POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS},
   year = {2012}
}

TY  - JOUR
ID  - 1169981
AU  - Kubáň, Petr - Kobrin, Eeva-Gerda - Mihkel, Kaljurand
PY  - 2012
TI  - POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS
PB  - Ústav analytické chemie AV ČR, v. v. i
CY  - Brno
SN  - 9788090495913
KW  - Exhaled breath condensate
KW  - capillary electrophoresis
KW  - point of care diagnostics
N2  - Exhaled breath condensate has been analyzed for its ionic content by capillary electrophoresis with capacitively coupled contactless conductometric detection. Two devices for exhaled breath condensate collection were compared. These include a tube-in-tube cooled sampler and a simple zip-lock bag. The devices allow collection of small volumes (100-200 mu L) of exhaled breath condensate in less than 2 min. A method for quick (less than 3 min) simultaneous determination of inorganic cations, inorganic anions and organic anions from the samples using dual-opposite end injection principle with a short fused silica capillary (35 cm, 50 mu m i.d.) was optimized with final background electrolyte composition of 20 mM 2-(N-morpholino) ethanesulfonic acid, 20 mM L-histidine, 30 mu M cetyltrimethylammonium bromide and 2 mM 18-crown-6 was used. It has been shown that changes of nitrite could be observed in acute inflammation of upper airways and in person with diagnosed mild chronic obstructive pulmonary disease, while the changes of other ions could also be observed.
ER  -

KUBÁŇ, Petr, Eeva-Gerda KOBRIN and Kaljurand MIHKEL. POTENTIAL OF EXHALED BREATH CONDENSATE ANALYSIS IN POINT OF CARE DIGNOSTICS. In Foret, F; Krenkova, J; Guttman, A; Kleparnik, K; Bocek, P. \textit{CECE 2012: 9TH INTERNATIONAL INTERDISCIPLINARY MEETING ON BIOANALYSIS}. Brno: Ústav analytické chemie AV ČR, v. v. i, 2012, p.~128-133. ISBN~978-80-904959-1-3.

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