BALINTOVÁ, Jana, Medard PLUCNARA, Pavlína VIDLÁKOVÁ, Radek POHL, Luděk HAVRAN, Miroslav FOJTA and Michal HOCEK. Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases. Chemistry - A European Journal. Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA, 2013, vol. 19, No 38, p. 12720–12731. ISSN 0947-6539. Available from: https://dx.doi.org/10.1002/chem.201301868.
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Basic information
Original name Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
Authors BALINTOVÁ, Jana (703 Slovakia), Medard PLUCNARA (203 Czech Republic), Pavlína VIDLÁKOVÁ (203 Czech Republic), Radek POHL (203 Czech Republic), Luděk HAVRAN (203 Czech Republic), Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution) and Michal HOCEK (203 Czech Republic).
Edition Chemistry - A European Journal, Weinheim, WILEY-VCH Verlag GmbH & Co. KGaA, 2013, 0947-6539.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.696
RIV identification code RIV/00216224:14740/13:00067004
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1002/chem.201301868
UT WoS 000324316300025
Keywords (in Czech) DNApolymeráza; elektrochemie; nukleosid trifosfát; sekvenování; voltametrie
Keywords in English DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry
Tags DNA, electrochemical detection, rivok, Voltammetry
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 25/4/2014 20:37.
Abstract
Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
Links
GBP206/12/G151, research and development projectName: Centrum nových přístupů k bioanalýze a molekulární diagnostice
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