Benzofurazane as a New Redox Label for Electrochemical
Detection of DNA: Towards Multipotential Redox Coding of DNA
Bases

J 2013

Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

BALINTOVÁ, Jana, Medard PLUCNARA, Pavlína VIDLÁKOVÁ, Radek POHL, Luděk HAVRAN et. al.

Basic information

Original name

Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

Authors

BALINTOVÁ, Jana (703 Slovakia), Medard PLUCNARA (203 Czech Republic), Pavlína VIDLÁKOVÁ (203 Czech Republic), Radek POHL (203 Czech Republic), Luděk HAVRAN (203 Czech Republic), Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution) and Michal HOCEK (203 Czech Republic)

Edition

Chemistry - A European Journal, Weinheim, WILEY-VCH Verlag GmbH & Co. KGaA, 2013, 0947-6539

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.696

RIV identification code

RIV/00216224:14740/13:00067004

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/chem.201301868

UT WoS

000324316300025

Keywords (in Czech)

DNApolymeráza; elektrochemie; nukleosid trifosfát; sekvenování; voltametrie

Keywords in English

DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry

Abstract

V originále

Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.

Links

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

BALINTOVÁ, Jana, Medard PLUCNARA, Pavlína VIDLÁKOVÁ, Radek POHL, Luděk HAVRAN, Miroslav FOJTA and Michal HOCEK. Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases. Chemistry - A European Journal. Weinheim: WILEY-VCH Verlag GmbH & Co. KGaA, 2013, vol. 19, No 38, p. 12720–12731. ISSN 0947-6539. Available from: https://dx.doi.org/10.1002/chem.201301868.

@article{1169994,
   author = {Balintová, Jana and Plucnara, Medard and Vidláková, Pavlína and Pohl, Radek and Havran, Luděk and Fojta, Miroslav and Hocek, Michal},
   article_location = {Weinheim},
   article_number = {38},
   doi = {http://dx.doi.org/10.1002/chem.201301868},
   keywords = {DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry},
   language = {eng},
   issn = {0947-6539},
   journal = {Chemistry - A European Journal},
   title = {Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04},
   volume = {19},
   year = {2013}
}

TY  - JOUR
ID  - 1169994
AU  - Balintová, Jana - Plucnara, Medard - Vidláková, Pavlína - Pohl, Radek - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
PY  - 2013
TI  - Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
JF  - Chemistry - A European Journal
VL  - 19
IS  - 38
SP  - 12720–12731
EP  - 12720–12731
PB  - WILEY-VCH Verlag GmbH & Co. KGaA
SN  - 09476539
KW  - DNA polymerase
KW  - electrochemistry
KW  - nucleoside triphosphates
KW  - sequencing
KW  - voltammetry
UR  - http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
L2  - http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
N2  - Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
ER  -

BALINTOVÁ, Jana, Medard PLUCNARA, Pavlína VIDLÁKOVÁ, Radek POHL, Luděk HAVRAN, Miroslav FOJTA and Michal HOCEK. Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases. \textit{Chemistry - A European Journal}. Weinheim: WILEY-VCH Verlag GmbH \&{} Co. KGaA, 2013, vol.~19, No~38, p.~12720–12731. ISSN~0947-6539. Available from: https://dx.doi.org/10.1002/chem.201301868.

Detailed Information on Publication Record