The principle building blocks of fully synthetic boron cluster compounds are electron deficient chemical clusters, which consist only of boron and hydrogen atoms and their derivatives are characterized by exo-skeletal substituents. Unique medical promises of these compounds and their use as biomarkers cause the need for systematic investigation of their physico-chemical properties, particularly those utilizable in efficient analytical methods suitable for their characterization and determination, including electrochemical activity of the boron cluster species in water.