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@article{1170127,
author = {Hofelmann, D. and di Benedetto, B. and Azad, S. C. and Micale, Vincenzo and Wotjak, C.T. and Rammes, G.},
article_location = {Amsterdam},
article_number = {Aug},
doi = {http://dx.doi.org/10.1016/j.brainres.2013.06.011},
keywords = {CB1; 5-HT3; Amygdala; Fear conditioning; Slice; IPSC},
language = {eng},
issn = {0006-8993},
journal = {Brain Research},
title = {Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments},
url = {http://www.sciencedirect.com/science/article/pii/S0006899313008627#},
volume = {1527},
year = {2013}
}
TY - JOUR
ID - 1170127
AU - Hofelmann, D. - di Benedetto, B. - Azad, S. C. - Micale, Vincenzo - Wotjak, C.T. - Rammes, G.
PY - 2013
TI - Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments
JF - Brain Research
VL - 1527
IS - Aug
SP - 47-56
EP - 47-56
PB - Elsevier
SN - 00068993
KW - CB1
KW - 5-HT3
KW - Amygdala
KW - Fear conditioning
KW - Slice
KW - IPSC
UR - http://www.sciencedirect.com/science/article/pii/S0006899313008627#
L2 - http://www.sciencedirect.com/science/article/pii/S0006899313008627#
N2 - Both the serotonergic and the endocannabinoid system play a major role in mediating fear and anxiety. In the basolateral amygdala (BLA) it has been shown that the cannabinoid receptor 1 (CB1) is highly co-expressed with 5-HT3 receptors on GABAergic intemeurons suggesting that 5-HT3 receptor activity modulates CB1-mediated effects on inhibitory synaptic transmission. In the present study, we investigated the possible interactions of CB1 and 5-HT3-mediated neuronal processes in the BLA using electrophysiological and behavioural approaches. Whole-cell patch-clamp recordings were performed in coronal brain slices of mice. Electric stimuli were delivered to the lateral amygdala to evoke GABA(A) receptor-mediated inhibitory postsynaptic currents (GABA(A)-eIPSCs) in the BLA. The induction of LTDi, a CB1-mediated depression of inhibitory synaptic transmission, was neither affected by the 5-HT3 antagonists ondansetron (OND; 20 mu M) and tropisetron (Trop; 50 nM) nor by the 5-HT3 agonists SR57227A (10 mu M). In auditory fear conditioning tests, mice treated with SR57227A (3.0 mg/kg i.p.) showed sustained freezing, whereas treatment with Trop (1.0 mg/kg i.p.) decreased the expression of conditioned fear. These effects were overruled by the CB1 antagonist rimonabant (RIM; 3.0 mg/kg), which caused increased freezing with or without co-treatment with Trop. In summary, these experiments do not support a functional interaction between CB1 and 5-HT3 receptors at the level of GABA neurotransmission in the BLA nor in terms of fear regulation. (C) 2013 Elsevier B.V. All rights reserved.
ER -
HOFELMANN, D., B. DI BENEDETTO, S. C. AZAD, Vincenzo MICALE, C.T. WOTJAK a G. RAMMES. Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments. Online. \textit{Brain Research}. Amsterdam: Elsevier, 2013, roč.~1527, Aug, s.~47-56. ISSN~0006-8993. Dostupné z: https://dx.doi.org/10.1016/j.brainres.2013.06.011. [citováno 2024-04-23]
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