Lack of interaction of endocannabinoids and 5-HT3
neurotransmission in associative fear circuits of the amygdala:
Evidence from electrophysiological and behavioural experiments

J 2013

Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments

HOFELMANN, D., B. DI BENEDETTO, S. C. AZAD, Vincenzo MICALE, C.T. WOTJAK et. al.

Basic information

Original name

Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments

Authors

HOFELMANN, D. (276 Germany), B. DI BENEDETTO (276 Germany), S. C. AZAD (276 Germany), Vincenzo MICALE (380 Italy, guarantor, belonging to the institution), C.T. WOTJAK (276 Germany) and G. RAMMES (276 Germany)

Edition

Brain Research, Amsterdam, Elsevier, 2013, 0006-8993

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.828

RIV identification code

RIV/00216224:14740/13:00072700

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.brainres.2013.06.011

UT WoS

000324225400005

Keywords in English

CB1; 5-HT3; Amygdala; Fear conditioning; Slice; IPSC

Abstract

V originále

Both the serotonergic and the endocannabinoid system play a major role in mediating fear and anxiety. In the basolateral amygdala (BLA) it has been shown that the cannabinoid receptor 1 (CB1) is highly co-expressed with 5-HT3 receptors on GABAergic intemeurons suggesting that 5-HT3 receptor activity modulates CB1-mediated effects on inhibitory synaptic transmission. In the present study, we investigated the possible interactions of CB1 and 5-HT3-mediated neuronal processes in the BLA using electrophysiological and behavioural approaches. Whole-cell patch-clamp recordings were performed in coronal brain slices of mice. Electric stimuli were delivered to the lateral amygdala to evoke GABA(A) receptor-mediated inhibitory postsynaptic currents (GABA(A)-eIPSCs) in the BLA. The induction of LTDi, a CB1-mediated depression of inhibitory synaptic transmission, was neither affected by the 5-HT3 antagonists ondansetron (OND; 20 mu M) and tropisetron (Trop; 50 nM) nor by the 5-HT3 agonists SR57227A (10 mu M). In auditory fear conditioning tests, mice treated with SR57227A (3.0 mg/kg i.p.) showed sustained freezing, whereas treatment with Trop (1.0 mg/kg i.p.) decreased the expression of conditioned fear. These effects were overruled by the CB1 antagonist rimonabant (RIM; 3.0 mg/kg), which caused increased freezing with or without co-treatment with Trop. In summary, these experiments do not support a functional interaction between CB1 and 5-HT3 receptors at the level of GABA neurotransmission in the BLA nor in terms of fear regulation. (C) 2013 Elsevier B.V. All rights reserved.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

HOFELMANN, D., B. DI BENEDETTO, S. C. AZAD, Vincenzo MICALE, C.T. WOTJAK and G. RAMMES. Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments. Brain Research. Amsterdam: Elsevier, 2013, vol. 1527, Aug, p. 47-56. ISSN 0006-8993. Available from: https://dx.doi.org/10.1016/j.brainres.2013.06.011.

@article{1170127,
   author = {Hofelmann, D. and di Benedetto, B. and Azad, S. C. and Micale, Vincenzo and Wotjak, C.T. and Rammes, G.},
   article_location = {Amsterdam},
   article_number = {Aug},
   doi = {http://dx.doi.org/10.1016/j.brainres.2013.06.011},
   keywords = {CB1; 5-HT3; Amygdala; Fear conditioning; Slice; IPSC},
   language = {eng},
   issn = {0006-8993},
   journal = {Brain Research},
   title = {Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments},
   url = {http://www.sciencedirect.com/science/article/pii/S0006899313008627#},
   volume = {1527},
   year = {2013}
}

TY  - JOUR
ID  - 1170127
AU  - Hofelmann, D. - di Benedetto, B. - Azad, S. C. - Micale, Vincenzo - Wotjak, C.T. - Rammes, G.
PY  - 2013
TI  - Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments
JF  - Brain Research
VL  - 1527
IS  - Aug
SP  - 47-56
EP  - 47-56
PB  - Elsevier
SN  - 00068993
KW  - CB1
KW  - 5-HT3
KW  - Amygdala
KW  - Fear conditioning
KW  - Slice
KW  - IPSC
UR  - http://www.sciencedirect.com/science/article/pii/S0006899313008627#
L2  - http://www.sciencedirect.com/science/article/pii/S0006899313008627#
N2  - Both the serotonergic and the endocannabinoid system play a major role in mediating fear and anxiety. In the basolateral amygdala (BLA) it has been shown that the cannabinoid receptor 1 (CB1) is highly co-expressed with 5-HT3 receptors on GABAergic intemeurons suggesting that 5-HT3 receptor activity modulates CB1-mediated effects on inhibitory synaptic transmission. In the present study, we investigated the possible interactions of CB1 and 5-HT3-mediated neuronal processes in the BLA using electrophysiological and behavioural approaches. Whole-cell patch-clamp recordings were performed in coronal brain slices of mice. Electric stimuli were delivered to the lateral amygdala to evoke GABA(A) receptor-mediated inhibitory postsynaptic currents (GABA(A)-eIPSCs) in the BLA. The induction of LTDi, a CB1-mediated depression of inhibitory synaptic transmission, was neither affected by the 5-HT3 antagonists ondansetron (OND; 20 mu M) and tropisetron (Trop; 50 nM) nor by the 5-HT3 agonists SR57227A (10 mu M). In auditory fear conditioning tests, mice treated with SR57227A (3.0 mg/kg i.p.) showed sustained freezing, whereas treatment with Trop (1.0 mg/kg i.p.) decreased the expression of conditioned fear. These effects were overruled by the CB1 antagonist rimonabant (RIM; 3.0 mg/kg), which caused increased freezing with or without co-treatment with Trop. In summary, these experiments do not support a functional interaction between CB1 and 5-HT3 receptors at the level of GABA neurotransmission in the BLA nor in terms of fear regulation. (C) 2013 Elsevier B.V. All rights reserved.
ER  -

HOFELMANN, D., B. DI BENEDETTO, S. C. AZAD, Vincenzo MICALE, C.T. WOTJAK and G. RAMMES. Lack of interaction of endocannabinoids and 5-HT3 neurotransmission in associative fear circuits of the amygdala: Evidence from electrophysiological and behavioural experiments. \textit{Brain Research}. Amsterdam: Elsevier, 2013, vol.~1527, Aug, p.~47-56. ISSN~0006-8993. Available from: https://dx.doi.org/10.1016/j.brainres.2013.06.011.

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