VAN4 Encodes a Putative TRS120 That is Required for Normal Cell
Growth and Vein Development in Arabidopsis

J 2014

VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis

NARAMOTO, S., Tomasz NODZYNSKI, T. DAINOBU, H. TAKATSUKA, T. OKADA et. al.

Basic information

Original name

VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis

Authors

NARAMOTO, S. (392 Japan), Tomasz NODZYNSKI (616 Poland, belonging to the institution), T. DAINOBU (392 Japan), H. TAKATSUKA (392 Japan), T. OKADA (392 Japan), Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution) and H. FUKUDA (392 Japan)

Edition

PLANT AND CELL PHYSIOLOGY, Oxford, Oxford University Press, 2014, 0032-0781

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.931

RIV identification code

RIV/00216224:14740/14:00073545

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/pcp/pcu012

UT WoS

000334679500009

Keywords in English

Arabidopsis; Cell growth; Guanine nucleotide exchange factor (GEF); Leaf venation pattern; Vascular cell differentiation; Vesicle trafficking

Abstract

V originále

Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

Citovat

NARAMOTO, S., Tomasz NODZYNSKI, T. DAINOBU, H. TAKATSUKA, T. OKADA, Jiří FRIML and H. FUKUDA. VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis. PLANT AND CELL PHYSIOLOGY. Oxford: Oxford University Press, 2014, vol. 55, No 4, p. 750-763. ISSN 0032-0781. Available from: https://dx.doi.org/10.1093/pcp/pcu012.

@article{1173185,
   author = {Naramoto, S. and Nodzynski, Tomasz and Dainobu, T. and Takatsuka, H. and Okada, T. and Friml, Jiří and Fukuda, H.},
   article_location = {Oxford},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/pcp/pcu012},
   keywords = {Arabidopsis; Cell growth; Guanine nucleotide exchange factor (GEF); Leaf venation pattern; Vascular cell differentiation; Vesicle trafficking},
   language = {eng},
   issn = {0032-0781},
   journal = {PLANT AND CELL PHYSIOLOGY},
   title = {VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis},
   url = {http://pcp.oxfordjournals.org/content/55/4/750.long},
   volume = {55},
   year = {2014}
}

TY  - JOUR
ID  - 1173185
AU  - Naramoto, S. - Nodzynski, Tomasz - Dainobu, T. - Takatsuka, H. - Okada, T. - Friml, Jiří - Fukuda, H.
PY  - 2014
TI  - VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis
JF  - PLANT AND CELL PHYSIOLOGY
VL  - 55
IS  - 4
SP  - 750-763
EP  - 750-763
PB  - Oxford University Press
SN  - 00320781
KW  - Arabidopsis
KW  - Cell growth
KW  - Guanine nucleotide exchange factor (GEF)
KW  - Leaf venation pattern
KW  - Vascular cell differentiation
KW  - Vesicle trafficking
UR  - http://pcp.oxfordjournals.org/content/55/4/750.long
L2  - http://pcp.oxfordjournals.org/content/55/4/750.long
N2  - Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation.
ER  -

NARAMOTO, S., Tomasz NODZYNSKI, T. DAINOBU, H. TAKATSUKA, T. OKADA, Jiří FRIML and H. FUKUDA. VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis. \textit{PLANT AND CELL PHYSIOLOGY}. Oxford: Oxford University Press, 2014, vol.~55, No~4, p.~750-763. ISSN~0032-0781. Available from: https://dx.doi.org/10.1093/pcp/pcu012.

Detailed Information on Publication Record