Detailed Information on Publication Record

DAMBORSKÝ, Jiří, Zbyněk PROKOP, Táňa KOUDELÁKOVÁ, Veronika ŠTĚPÁNKOVÁ, Radka CHALOUPKOVÁ, Eva CHOVANCOVÁ, Jan BREZOVSKÝ and Artur Wiktor GORA. Method of Thermostabilization of a Protein And/or Stabilization Towards Organic Solvents. 2013.

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Basic information
Original name	Method of Thermostabilization of a Protein And/or Stabilization Towards Organic Solvents
Authors	DAMBORSKÝ, Jiří (203 Czech Republic, guarantor, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Táňa KOUDELÁKOVÁ (203 Czech Republic, belonging to the institution), Veronika ŠTĚPÁNKOVÁ (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Eva CHOVANCOVÁ (203 Czech Republic), Jan BREZOVSKÝ (203 Czech Republic, belonging to the institution) and Artur Wiktor GORA (616 Poland).
Edition	Number: US 8,580,932, Publisher: USTPO (Úřad pro patenty a ochranné známky USA), Owner's name: MU, 2013.

Other information
Original language	English
Type of outcome	Patent
Field of Study	10600 1.6 Biological sciences
Country of publisher	United States of America
Confidentiality degree	is not subject to a state or trade secret
RIV identification code	RIV/00216224:14310/13:00072736
Organization unit	Faculty of Science
Keywords in English	Thermostabilization; Protein Stabilization; Organic Solvents
Tags	AKR, rivok
Changed by	Changed by: prof. Mgr. Jiří Damborský, Dr., učo 1441. Changed: 25/3/2014 18:21.

Abstract
The present invention relates to a method of thermostabilization of a protein and/or stabilization of a protein towards organic solvents wherein the protein contains access routes, wherein at least one amino acid in the bottleneck of the access route of the protein is mutated. Preferably, the method comprises the steps of identification of amino acids forming the bottleneck of the access routes, wherein said amino acids control the exchange of the solvent between the buried protein core and the surrounding environment and/or in the packing of the amino acids inside the access route; modification of the amino acids determined in the previous step so that the packing of the amino acids inside the tunnel is improved and the access route prevents the access of the undesired solvent molecules to the protein core, while it allows the passage of the compounds necessary at the protein core to enable the protein to perform its biological function. Preferably, the method further includes the step of assessment of the result of the modification, wherein the enzyme is analyzed in the form of whole cells containing the expressed protein, crude extract, or partially purified or purified protein.

Abstract

The present invention relates to a method of thermostabilization of a protein and/or stabilization of a protein towards organic solvents wherein the protein contains access routes, wherein at least one amino acid in the bottleneck of the access route of the protein is mutated. Preferably, the method comprises the steps of identification of amino acids forming the bottleneck of the access routes, wherein said amino acids control the exchange of the solvent between the buried protein core and the surrounding environment and/or in the packing of the amino acids inside the access route; modification of the amino acids determined in the previous step so that the packing of the amino acids inside the tunnel is improved and the access route prevents the access of the undesired solvent molecules to the protein core, while it allows the passage of the compounds necessary at the protein core to enable the protein to perform its biological function. Preferably, the method further includes the step of assessment of the result of the modification, wherein the enzyme is analyzed in the form of whole cells containing the expressed protein, crude extract, or partially purified or purified protein.

Links
ED0001/01/01, research and development project	Name: CETOCOEN