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@proceedings{1173336,
author = {Táborský, Petr and Rájecký, Michal and Táborská, Eva and Gargallo, Raimundo},
booktitle = {European symposium on atomic spectrometry ESAS 2014 & 15th Czech-Slovak spectroscopic conference},
keywords = {benzo[c]phenanthridine alkaloids; DNA; fluorescence},
isbn = {978-80-905704-1-2},
title = {Fluorescent DNA probes based on phenantridine alkaloids},
year = {2014}
}
TY - CONF
ID - 1173336
AU - Táborský, Petr - Rájecký, Michal - Táborská, Eva - Gargallo, Raimundo
PY - 2014
TI - Fluorescent DNA probes based on phenantridine alkaloids
SN - 9788090570412
KW - benzo[c]phenanthridine alkaloids
KW - DNA
KW - fluorescence
N2 - Thanks to exceptional affinity of plant alkaloids to various biomolecules they can be used in analytical chemistry as a probes. Many isochinoline alkaloids are fluorescent and interact with DNA by intercalation. Phenantridine alkaloids such as sanguinarine, chelerytrine, chelirubine, chelilutine, sanguilutine and macarpine interact with double stranded DNA showing substantial changes in fluorescence emission. The response of luminescence intensity to low concentrations of double stranded DNA in presence of alkaloids was found to be linear and can be potentially used for quantification of DNA by means of luminescence spectrometry. For example, limit of detection (3σ method) calculated for alkaloid chelirubine was calculated to be 8 ng.mL -1 of DNA. Chelirubine and macarpine were even successfully used for fluorescent DNA-specific staining of living cells due to the lower toxicity. In conclusion, we have demonstrated that natural products, phenantridine alkaloids, can be used as alternative probes to synthetic DNA dyes such as ethidium bromide.
ER -
TÁBORSKÝ, Petr, Michal RÁJECKÝ, Eva TÁBORSKÁ and Raimundo GARGALLO. Fluorescent DNA probes based on phenantridine alkaloids. In \textit{European symposium on atomic spectrometry ESAS 2014 \&{} 15th Czech-Slovak spectroscopic conference}. 2014. ISBN~978-80-905704-1-2.
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