Acute, chronic and reproductive toxicity of complex
cyanobacterial blooms in Daphnia magna and the role of
microcystins

J 2014

Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins

SMUTNÁ, Marie, Pavel BABICA, Sergio JARQUE ORTIZ, Klára HILSCHEROVÁ, Blahoslav MARŠÁLEK et. al.

Basic information

Original name

Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins

Authors

SMUTNÁ, Marie (203 Czech Republic, belonging to the institution), Pavel BABICA (203 Czech Republic, belonging to the institution), Sergio JARQUE ORTIZ (724 Spain, belonging to the institution), Klára HILSCHEROVÁ (203 Czech Republic, belonging to the institution), Blahoslav MARŠÁLEK (203 Czech Republic, belonging to the institution), Maher HAEBA (434 Libya, belonging to the institution) and Luděk BLÁHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Toxicon, OXFORD, Elsevier Science, 2014, 0041-0101

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 2.492

RIV identification code

RIV/00216224:14310/14:00073577

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.toxicon.2013.12.009

UT WoS

000332444100002

Keywords in English

Cyanotoxin; Reproduction; Cyanobacterial bloom; Daphnia magna; Microcystin

Abstract

V originále

Ecotoxicity of toxins from cyanobacteria such as microcystins has been studied extensively, little is known about the risks they pose in the wild, i.e. within complex biomasses. In this work, crustaceans (Daphnia magna) were exposed to varying concentrations (0-405 mg d.w L-1) of eight complex cyanobacterial water bloom samples in a series of acute (48 h) and chronic (21 day) toxicity experiments. Further acute and chronic exposure assays were performed using aqueous extracts of the crude biomass samples and two fractions prepared by solid phase extraction (SPE) of the aqueous extracts. High acute toxicity was observed for 6 of the 8 crude biomass samples. Chronic exposure assays were performed using one complex biomass sample and its various subsamples/fractions. The complex biomass, the crude aqueous extract, and the microcystin-free SPE permeate all elicited similar and significant lethal effects, with LC50 values of around 35.6 mg biomass d.w L-1 after 21 days. The cyanobacterial biomass samples also affected reproductive health, significantly increasing the time to the first brood (LOEC = 45 mg center dot d.w L-1 exposure) and inhibiting fecundity by 50% at 15 mg d.w L-1. Conversely, the microcystin-containing C18-SPE eluate fraction had only weak effects in the chronic assay. These results indicate that cyanobacterial water blooms are highly toxic to zooplankton (both acutely and chronically) at environmentally relevant concentrations. However, the effects observed in the acute and chronic assays were independent of the samples' microcystin contents. Our results thus point out the importance of other cyanobacterial components such as lipopolysaccharides, various peptides and depsipeptides, polar alkaloid metabolites or other unidentified metabolites in the overall ecotoxicity of complex cyanobacterial blooms.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP503/12/0553, research and development project

Name: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Czech Science Foundation

Citovat

SMUTNÁ, Marie, Pavel BABICA, Sergio JARQUE ORTIZ, Klára HILSCHEROVÁ, Blahoslav MARŠÁLEK, Maher HAEBA and Luděk BLÁHA. Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins. Toxicon. OXFORD: Elsevier Science, 2014, vol. 79, march, p. 11-18. ISSN 0041-0101. Available from: https://dx.doi.org/10.1016/j.toxicon.2013.12.009.

@article{1174154,
   author = {Smutná, Marie and Babica, Pavel and Jarque Ortiz, Sergio and Hilscherová, Klára and Maršálek, Blahoslav and Haeba, Maher and Bláha, Luděk},
   article_location = {OXFORD},
   article_number = {march},
   doi = {http://dx.doi.org/10.1016/j.toxicon.2013.12.009},
   keywords = {Cyanotoxin; Reproduction; Cyanobacterial bloom; Daphnia magna; Microcystin},
   language = {eng},
   issn = {0041-0101},
   journal = {Toxicon},
   title = {Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins},
   volume = {79},
   year = {2014}
}

TY  - JOUR
ID  - 1174154
AU  - Smutná, Marie - Babica, Pavel - Jarque Ortiz, Sergio - Hilscherová, Klára - Maršálek, Blahoslav - Haeba, Maher - Bláha, Luděk
PY  - 2014
TI  - Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins
JF  - Toxicon
VL  - 79
IS  - march
SP  - 11-18
EP  - 11-18
PB  - Elsevier Science
SN  - 00410101
KW  - Cyanotoxin
KW  - Reproduction
KW  - Cyanobacterial bloom
KW  - Daphnia magna
KW  - Microcystin
N2  - Ecotoxicity of toxins from cyanobacteria such as microcystins has been studied extensively, little is known about the risks they pose in the wild, i.e. within complex biomasses. In this work, crustaceans (Daphnia magna) were exposed to varying concentrations (0-405 mg d.w L-1) of eight complex cyanobacterial water bloom samples in a series of acute (48 h) and chronic (21 day) toxicity experiments. Further acute and chronic exposure assays were performed using aqueous extracts of the crude biomass samples and two fractions prepared by solid phase extraction (SPE) of the aqueous extracts. High acute toxicity was observed for 6 of the 8 crude biomass samples. Chronic exposure assays were performed using one complex biomass sample and its various subsamples/fractions. The complex biomass, the crude aqueous extract, and the microcystin-free SPE permeate all elicited similar and significant lethal effects, with LC50 values of around 35.6 mg biomass d.w L-1 after 21 days. The cyanobacterial biomass samples also affected reproductive health, significantly increasing the time to the first brood (LOEC = 45 mg center dot d.w L-1 exposure) and inhibiting fecundity by 50% at 15 mg d.w L-1. Conversely, the microcystin-containing C18-SPE eluate fraction had only weak effects in the chronic assay. These results indicate that cyanobacterial water blooms are highly toxic to zooplankton (both acutely and chronically) at environmentally relevant concentrations. However, the effects observed in the acute and chronic assays were independent of the samples' microcystin contents. Our results thus point out the importance of other cyanobacterial components such as lipopolysaccharides, various peptides and depsipeptides, polar alkaloid metabolites or other unidentified metabolites in the overall ecotoxicity of complex cyanobacterial blooms.
ER  -

SMUTNÁ, Marie, Pavel BABICA, Sergio JARQUE ORTIZ, Klára HILSCHEROVÁ, Blahoslav MARŠÁLEK, Maher HAEBA and Luděk BLÁHA. Acute, chronic and reproductive toxicity of complex cyanobacterial blooms in Daphnia magna and the role of microcystins. \textit{Toxicon}. OXFORD: Elsevier Science, 2014, vol.~79, march, p.~11-18. ISSN~0041-0101. Available from: https://dx.doi.org/10.1016/j.toxicon.2013.12.009.

Detailed Information on Publication Record