Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne
asmulti-redox centre DNA label at mercury meniscus modified
silversolid amalgam electrode

J 2014

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK et. al.

Basic information

Original name

Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode

Authors

DAŇHEL, Aleš (203 Czech Republic), Veronika RAINDLOVA (203 Czech Republic), Luděk HAVRAN (203 Czech Republic), Hana PIVOŇKOVÁ (203 Czech Republic), Michal HOCEK (203 Czech Republic) and Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Electrochimica Acta, OXFORD, Elsevier Science Ltd, 2014, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.504

RIV identification code

RIV/00216224:14740/14:00073589

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2013.09.147

UT WoS

000336340900016

Keywords in English

DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry

Abstract

V originále

A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.

Links

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK and Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. Electrochimica Acta. OXFORD: Elsevier Science Ltd, 2014, vol. 126, SI, p. 122-131. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2013.09.147.

@article{1179581,
   author = {Daňhel, Aleš and Raindlova, Veronika and Havran, Luděk and Pivoňková, Hana and Hocek, Michal and Fojta, Miroslav},
   article_location = {OXFORD},
   article_number = {SI},
   doi = {http://dx.doi.org/10.1016/j.electacta.2013.09.147},
   keywords = {DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode},
   url = {http://dx.doi.org/10.1016/j.electacta.2013.09.147},
   volume = {126},
   year = {2014}
}

TY  - JOUR
ID  - 1179581
AU  - Daňhel, Aleš - Raindlova, Veronika - Havran, Luděk - Pivoňková, Hana - Hocek, Michal - Fojta, Miroslav
PY  - 2014
TI  - Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
JF  - Electrochimica Acta
VL  - 126
IS  - SI
SP  - 122-131
EP  - 122-131
PB  - Elsevier Science Ltd
SN  - 00134686
KW  - DNA
KW  - Redox labeling
KW  - 2_4-Dinitrophenylhydrazine
KW  - Silver solid amalgam electrode
KW  - Voltammetry
UR  - http://dx.doi.org/10.1016/j.electacta.2013.09.147
L2  - http://dx.doi.org/10.1016/j.electacta.2013.09.147
N2  - A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.
ER  -

DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK and Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. \textit{Electrochimica Acta}. OXFORD: Elsevier Science Ltd, 2014, vol.~126, SI, p.~122-131. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2013.09.147.

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