DAŇHEL, Aleš, Veronika RAINDLOVA, Luděk HAVRAN, Hana PIVOŇKOVÁ, Michal HOCEK and Miroslav FOJTA. Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode. Electrochimica Acta. OXFORD: Elsevier Science Ltd, 2014, vol. 126, SI, p. 122-131. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2013.09.147.
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Basic information
Original name Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
Authors DAŇHEL, Aleš (203 Czech Republic), Veronika RAINDLOVA (203 Czech Republic), Luděk HAVRAN (203 Czech Republic), Hana PIVOŇKOVÁ (203 Czech Republic), Michal HOCEK (203 Czech Republic) and Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution).
Edition Electrochimica Acta, OXFORD, Elsevier Science Ltd, 2014, 0013-4686.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10405 Electrochemistry
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.504
RIV identification code RIV/00216224:14740/14:00073589
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.electacta.2013.09.147
UT WoS 000336340900016
Keywords in English DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry
Tags DNA, kontrola MP, rivok, Voltammetry
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 11/3/2015 08:30.
Abstract
A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications.
Links
GBP206/12/G151, research and development projectName: Centrum nových přístupů k bioanalýze a molekulární diagnostice
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