BARTOSIK, Martin, Miroslav FOJTA and Emil PALECEK. Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA. Electrochimica Acta. Oxford: Pergamon-Elsevier Science, 2012, vol. 78, Sep, p. 75-81. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2012.05.115. |
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@article{1179810, author = {Bartosik, Martin and Fojta, Miroslav and Palecek, Emil}, article_location = {Oxford}, article_number = {Sep}, doi = {http://dx.doi.org/10.1016/j.electacta.2012.05.115}, keywords = {DNA methylation; 5-Methylcytosine analysis; Sodium bisulfite; Mercury electrode; Solid amalgam electrode}, language = {eng}, issn = {0013-4686}, journal = {Electrochimica Acta}, title = {Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA}, volume = {78}, year = {2012} }
TY - JOUR ID - 1179810 AU - Bartosik, Martin - Fojta, Miroslav - Palecek, Emil PY - 2012 TI - Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA JF - Electrochimica Acta VL - 78 IS - Sep SP - 75-81 EP - 75-81 PB - Pergamon-Elsevier Science SN - 00134686 KW - DNA methylation KW - 5-Methylcytosine analysis KW - Sodium bisulfite KW - Mercury electrode KW - Solid amalgam electrode N2 - DNA methylation is an important epigenetic event playing crucial roles in physiologic and pathologic processes. We show that methylation of cytosine (C) residues in DNA can be easily detected electrochemically using mercury or solid amalgam electrodes. Reduction peaks of untreated single-stranded methylated and non-methylated oligodeoxynucleotides (ODN) do not significantly differ. Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand. 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step should be omitted because uracil sulfonation improves the resolution of C from mC. By combining DNA bisulfite treatment with square wave voltammetry. DNA methylation can be determined quantitatively at nanomolar and subnamolar ODN concentrations. ER -
BARTOSIK, Martin, Miroslav FOJTA and Emil PALECEK. Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA. \textit{Electrochimica Acta}. Oxford: Pergamon-Elsevier Science, 2012, vol.~78, Sep, p.~75-81. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2012.05.115.
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