Electrochemical detection of 5-methylcytosine in
bisulfite-treated DNA

J 2012

Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA

BARTOSIK, Martin, Miroslav FOJTA and Emil PALECEK

Basic information

Original name

Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA

Authors

BARTOSIK, Martin (203 Czech Republic), Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution) and Emil PALECEK (203 Czech Republic)

Edition

Electrochimica Acta, Oxford, Pergamon-Elsevier Science, 2012, 0013-4686

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.777

RIV identification code

RIV/00216224:14740/12:00073027

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.electacta.2012.05.115

UT WoS

000308259500010

Keywords in English

DNA methylation; 5-Methylcytosine analysis; Sodium bisulfite; Mercury electrode; Solid amalgam electrode

Abstract

V originále

DNA methylation is an important epigenetic event playing crucial roles in physiologic and pathologic processes. We show that methylation of cytosine (C) residues in DNA can be easily detected electrochemically using mercury or solid amalgam electrodes. Reduction peaks of untreated single-stranded methylated and non-methylated oligodeoxynucleotides (ODN) do not significantly differ. Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand. 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step should be omitted because uracil sulfonation improves the resolution of C from mC. By combining DNA bisulfite treatment with square wave voltammetry. DNA methylation can be determined quantitatively at nanomolar and subnamolar ODN concentrations.

Citovat

BARTOSIK, Martin, Miroslav FOJTA and Emil PALECEK. Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA. Electrochimica Acta. Oxford: Pergamon-Elsevier Science, 2012, vol. 78, Sep, p. 75-81. ISSN 0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2012.05.115.

@article{1179810,
   author = {Bartosik, Martin and Fojta, Miroslav and Palecek, Emil},
   article_location = {Oxford},
   article_number = {Sep},
   doi = {http://dx.doi.org/10.1016/j.electacta.2012.05.115},
   keywords = {DNA methylation; 5-Methylcytosine analysis; Sodium bisulfite; Mercury electrode; Solid amalgam electrode},
   language = {eng},
   issn = {0013-4686},
   journal = {Electrochimica Acta},
   title = {Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA},
   volume = {78},
   year = {2012}
}

TY  - JOUR
ID  - 1179810
AU  - Bartosik, Martin - Fojta, Miroslav - Palecek, Emil
PY  - 2012
TI  - Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA
JF  - Electrochimica Acta
VL  - 78
IS  - Sep
SP  - 75-81
EP  - 75-81
PB  - Pergamon-Elsevier Science
SN  - 00134686
KW  - DNA methylation
KW  - 5-Methylcytosine analysis
KW  - Sodium bisulfite
KW  - Mercury electrode
KW  - Solid amalgam electrode
N2  - DNA methylation is an important epigenetic event playing crucial roles in physiologic and pathologic processes. We show that methylation of cytosine (C) residues in DNA can be easily detected electrochemically using mercury or solid amalgam electrodes. Reduction peaks of untreated single-stranded methylated and non-methylated oligodeoxynucleotides (ODN) do not significantly differ. Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand. 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step should be omitted because uracil sulfonation improves the resolution of C from mC. By combining DNA bisulfite treatment with square wave voltammetry. DNA methylation can be determined quantitatively at nanomolar and subnamolar ODN concentrations.
ER  -

BARTOSIK, Martin, Miroslav FOJTA and Emil PALECEK. Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA. \textit{Electrochimica Acta}. Oxford: Pergamon-Elsevier Science, 2012, vol.~78, Sep, p.~75-81. ISSN~0013-4686. Available from: https://dx.doi.org/10.1016/j.electacta.2012.05.115.

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