BARTOSIK, Martin, Miroslav FOJTA and Emil PALECEK. Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA. Electrochimica Acta. Oxford: Pergamon-Elsevier Science, vol. 78, Sep, p. 75-81. ISSN 0013-4686. doi:10.1016/j.electacta.2012.05.115. 2012.
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Basic information
Original name Electrochemical detection of 5-methylcytosine in bisulfite-treated DNA
Authors BARTOSIK, Martin (203 Czech Republic), Miroslav FOJTA (203 Czech Republic, guarantor, belonging to the institution) and Emil PALECEK (203 Czech Republic).
Edition Electrochimica Acta, Oxford, Pergamon-Elsevier Science, 2012, 0013-4686.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10610 Biophysics
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.777
RIV identification code RIV/00216224:14740/12:00073027
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.electacta.2012.05.115
UT WoS 000308259500010
Keywords in English DNA methylation; 5-Methylcytosine analysis; Sodium bisulfite; Mercury electrode; Solid amalgam electrode
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 13/4/2014 10:30.
Abstract
DNA methylation is an important epigenetic event playing crucial roles in physiologic and pathologic processes. We show that methylation of cytosine (C) residues in DNA can be easily detected electrochemically using mercury or solid amalgam electrodes. Reduction peaks of untreated single-stranded methylated and non-methylated oligodeoxynucleotides (ODN) do not significantly differ. Using DNA bisulfite treatment, reducible Cs are transformed into nonreducible uracil residues, strongly decreasing square wave voltammetric C reduction peaks. On the other hand. 5-methylcytosine (mC) residues resist the bisulfite treatment and display almost unchanged reduction peak. Desulfonation step should be omitted because uracil sulfonation improves the resolution of C from mC. By combining DNA bisulfite treatment with square wave voltammetry. DNA methylation can be determined quantitatively at nanomolar and subnamolar ODN concentrations.
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