Circular dichroism and conformational polymorphism of DNA

J 2009

Circular dichroism and conformational polymorphism of DNA

KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK and Michaela VORLÍČKOVÁ

Basic information

Original name

Circular dichroism and conformational polymorphism of DNA

Authors

KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK and Michaela VORLÍČKOVÁ

Edition

Nucleic Acids Research, Oxford, Oxford Press, 2009, 0305-1048

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 7.479

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/nar/gkp026

UT WoS

000265097400008

Keywords in English

GUANINE-GUANINE STACKING; HUMAN TELOMERIC SEQUENCE; ADENINE REPEAT STRANDS; DOUBLE-HELICAL DNA; G-QUADRUPLEX; NUCLEIC-ACID; POTASSIUM SOLUTION; CRYSTAL-STRUCTURE; MELTING CURVES; SYNTHETIC DNA

Abstract

V originále

Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA.

Citovat

KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK and Michaela VORLÍČKOVÁ. Circular dichroism and conformational polymorphism of DNA. Nucleic Acids Research. Oxford: Oxford Press, 2009, vol. 37, No 6, p. 1713-1725. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkp026.

@article{1180214,
   author = {Kypr, Jaroslav and Kejnovská, Iva and Renčiuk, Daniel and Vorlíčková, Michaela},
   article_location = {Oxford},
   article_number = {6},
   doi = {http://dx.doi.org/10.1093/nar/gkp026},
   keywords = {GUANINE-GUANINE STACKING; HUMAN TELOMERIC SEQUENCE; ADENINE REPEAT STRANDS; DOUBLE-HELICAL DNA; G-QUADRUPLEX; NUCLEIC-ACID; POTASSIUM SOLUTION; CRYSTAL-STRUCTURE; MELTING CURVES; SYNTHETIC DNA},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {Circular dichroism and conformational polymorphism of DNA},
   volume = {37},
   year = {2009}
}

TY  - JOUR
ID  - 1180214
AU  - Kypr, Jaroslav - Kejnovská, Iva - Renčiuk, Daniel - Vorlíčková, Michaela
PY  - 2009
TI  - Circular dichroism and conformational polymorphism of DNA
JF  - Nucleic Acids Research
VL  - 37
IS  - 6
SP  - 1713-1725
EP  - 1713-1725
PB  - Oxford Press
SN  - 03051048
KW  - GUANINE-GUANINE STACKING
KW  - HUMAN TELOMERIC SEQUENCE
KW  - ADENINE REPEAT STRANDS
KW  - DOUBLE-HELICAL DNA
KW  - G-QUADRUPLEX
KW  - NUCLEIC-ACID
KW  - POTASSIUM SOLUTION
KW  - CRYSTAL-STRUCTURE
KW  - MELTING CURVES
KW  - SYNTHETIC DNA
N2  - Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA.
ER  -

KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK and Michaela VORLÍČKOVÁ. Circular dichroism and conformational polymorphism of DNA. \textit{Nucleic Acids Research}. Oxford: Oxford Press, 2009, vol.~37, No~6, p.~1713-1725. ISSN~0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkp026.

Detailed Information on Publication Record