KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK and Michaela VORLÍČKOVÁ. Circular dichroism and conformational polymorphism of DNA. Nucleic Acids Research. Oxford: Oxford Press, 2009, vol. 37, No 6, p. 1713-1725. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkp026.
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Basic information
Original name Circular dichroism and conformational polymorphism of DNA
Authors KYPR, Jaroslav, Iva KEJNOVSKÁ, Daniel RENČIUK and Michaela VORLÍČKOVÁ.
Edition Nucleic Acids Research, Oxford, Oxford Press, 2009, 0305-1048.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 7.479
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/nar/gkp026
UT WoS 000265097400008
Keywords in English GUANINE-GUANINE STACKING; HUMAN TELOMERIC SEQUENCE; ADENINE REPEAT STRANDS; DOUBLE-HELICAL DNA; G-QUADRUPLEX; NUCLEIC-ACID; POTASSIUM SOLUTION; CRYSTAL-STRUCTURE; MELTING CURVES; SYNTHETIC DNA
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Changed by Changed by: Olga Křížová, učo 56639. Changed: 14/4/2014 09:49.
Abstract
Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA.
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