Strong population genetic structuring in an annual fish,
Nothobranchius furzeri, suggests multiple savannah refugia in
southern Mozambique

J 2013

Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique

BARTÁKOVÁ, Veronika, Martin REICHARD, Karel JANKO, Matej POLAČIK, Radim BLAŽEK et. al.

Basic information

Original name

Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique

Authors

BARTÁKOVÁ, Veronika (203 Czech Republic, guarantor, belonging to the institution), Martin REICHARD (203 Czech Republic), Karel JANKO (203 Czech Republic), Matej POLAČIK (203 Czech Republic), Radim BLAŽEK (203 Czech Republic), Kathrin REICHWALD (276 Germany), Alessandro CELLERINO (380 Italy) and Josef BRYJA (203 Czech Republic)

Edition

BMC Evolutionary Biology, BioMed Central, 2013, 1471-2148

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.407

RIV identification code

RIV/00216224:14310/13:00067064

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/1471-2148-13-196

UT WoS

000324749400003

Keywords in English

Temporary pool; Phylogeography; Population genetics; Cyprinodontiformes; Senescence; Pluvials; Pleistocene climate changes; Dispersal; Founder effect; Killifish

Abstract

V originále

Background: Intraspecific genetic variation of African fauna has been significantly affected by pronounced climatic fluctuations in Plio-Pleistocene, but, with the exception of large mammals, very limited empirical data on diversity of natural populations are available for savanna-dwelling animals. Nothobranchius furzeri is an annual fish from south-eastern Africa, inhabiting discrete temporary savannah pools outside main river alluvia. Their dispersal is limited and population processes affecting its genetic structure are likely a combination of those affecting terrestrial and aquatic taxa. N. furzeri is a model taxon in ageing research and several populations of known geographical origin are used in laboratory studies. Here, we analysed the genetic structure, diversity, historical demography and temporal patterns of divergence in natural populations of N. furzeri across its entire distribution range. Results: Genetic structure and historical demography of N. furzeri were analysed using a combination of mitochondrial (partial cytochrome b sequences, 687 bp) and nuclear (13 microsatellites) markers in 693 fish from 36 populations. Genetic markers consistently demonstrated strong population structuring and suggested two main genetic groups associated with river basins. The split was dated to the Pliocene (>2 Mya). The northern group inhabits savannah pools across the basin of the intermittent river Chefu in south-western Mozambique and eastern Zimbabwe. The southern group (from southernmost Mozambique) is subdivided, with the River Limpopo forming a barrier (maximum divergence time 1 Mya). A strong habitat fragmentation (isolated temporary pools) is reflected in significant genetic structuring even between adjacent pools, with a major influence of genetic drift and significant isolation-by-distance. Analysis of historical demography revealed that the expansion of both groups is ongoing, supported by frequent founder effects in marginal parts of the range and evidence of secondary contact between Chefu and Limpopo populations. Conclusions: We demonstrated: (1) ancient (pre-Pleistocene) divergence between the two main N. furzeri lineages, their recent secondary contact and lack of reproductive isolation; (2) important genetic structuring attributed to the fragmented nature of their environment and isolation-by-distance, suggesting that dispersal is limited, occurs over short distances and is not directly associated with river routes; (3) an apparent role of the River Limpopo as a barrier to dispersal and gene flow.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

BARTÁKOVÁ, Veronika, Martin REICHARD, Karel JANKO, Matej POLAČIK, Radim BLAŽEK, Kathrin REICHWALD, Alessandro CELLERINO and Josef BRYJA. Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique. BMC Evolutionary Biology. BioMed Central, 2013, vol. 13, No 196, p. 1-15. ISSN 1471-2148. Available from: https://dx.doi.org/10.1186/1471-2148-13-196.

@article{1180258,
   author = {Bartáková, Veronika and Reichard, Martin and Janko, Karel and Polačik, Matej and Blažek, Radim and Reichwald, Kathrin and Cellerino, Alessandro and Bryja, Josef},
   article_number = {196},
   doi = {http://dx.doi.org/10.1186/1471-2148-13-196},
   keywords = {Temporary pool; Phylogeography; Population genetics; Cyprinodontiformes; Senescence; Pluvials; Pleistocene climate changes; Dispersal; Founder effect; Killifish},
   language = {eng},
   issn = {1471-2148},
   journal = {BMC Evolutionary Biology},
   title = {Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique},
   volume = {13},
   year = {2013}
}

TY  - JOUR
ID  - 1180258
AU  - Bartáková, Veronika - Reichard, Martin - Janko, Karel - Polačik, Matej - Blažek, Radim - Reichwald, Kathrin - Cellerino, Alessandro - Bryja, Josef
PY  - 2013
TI  - Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique
JF  - BMC Evolutionary Biology
VL  - 13
IS  - 196
SP  - 1-15
EP  - 1-15
PB  - BioMed Central
SN  - 14712148
KW  - Temporary pool
KW  - Phylogeography
KW  - Population genetics
KW  - Cyprinodontiformes
KW  - Senescence
KW  - Pluvials
KW  - Pleistocene climate changes
KW  - Dispersal
KW  - Founder effect
KW  - Killifish
N2  - Background: Intraspecific genetic variation of African fauna has been significantly affected by pronounced climatic fluctuations in Plio-Pleistocene, but, with the exception of large mammals, very limited empirical data on diversity of natural populations are available for savanna-dwelling animals. Nothobranchius furzeri is an annual fish from south-eastern Africa, inhabiting discrete temporary savannah pools outside main river alluvia. Their dispersal is limited and population processes affecting its genetic structure are likely a combination of those affecting terrestrial and aquatic taxa. N. furzeri is a model taxon in ageing research and several populations of known geographical origin are used in laboratory studies. Here, we analysed the genetic structure, diversity, historical demography and temporal patterns of divergence in natural populations of N. furzeri across its entire distribution range. Results: Genetic structure and historical demography of N. furzeri were analysed using a combination of mitochondrial (partial cytochrome b sequences, 687 bp) and nuclear (13 microsatellites) markers in 693 fish from 36 populations. Genetic markers consistently demonstrated strong population structuring and suggested two main genetic groups associated with river basins. The split was dated to the Pliocene (>2 Mya). The northern group inhabits savannah pools across the basin of the intermittent river Chefu in south-western Mozambique and eastern Zimbabwe. The southern group (from southernmost Mozambique) is subdivided, with the River Limpopo forming a barrier (maximum divergence time 1 Mya). A strong habitat fragmentation (isolated temporary pools) is reflected in significant genetic structuring even between adjacent pools, with a major influence of genetic drift and significant isolation-by-distance. Analysis of historical demography revealed that the expansion of both groups is ongoing, supported by frequent founder effects in marginal parts of the range and evidence of secondary contact between Chefu and Limpopo populations. Conclusions: We demonstrated: (1) ancient (pre-Pleistocene) divergence between the two main N. furzeri lineages, their recent secondary contact and lack of reproductive isolation; (2) important genetic structuring attributed to the fragmented nature of their environment and isolation-by-distance, suggesting that dispersal is limited, occurs over short distances and is not directly associated with river routes; (3) an apparent role of the River Limpopo as a barrier to dispersal and gene flow.
ER  -

BARTÁKOVÁ, Veronika, Martin REICHARD, Karel JANKO, Matej POLAČIK, Radim BLAŽEK, Kathrin REICHWALD, Alessandro CELLERINO and Josef BRYJA. Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique. \textit{BMC Evolutionary Biology}. BioMed Central, 2013, vol.~13, No~196, p.~1-15. ISSN~1471-2148. Available from: https://dx.doi.org/10.1186/1471-2148-13-196.

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