TUDEK, Agnieszka, Odil Porrua FUERTE, Tomasz KABZINSKI, Michael LIDSCHREIBER, Karel KUBÍČEK, Andrea FOŘTOVÁ, Francoise LACROUTE, Štěpánka VAŇÁČOVÁ, Patrick CRAMER, Richard ŠTEFL a Domenico LIBRI. Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation. Molecular Cell. CAMBRIDGE: CELL PRESS, roč. 55, č. 3, s. 467-481. ISSN 1097-2765. doi:10.1016/j.molcel.2014.05.031. 2014. |
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@article{1182103, author = {Tudek, Agnieszka and Fuerte, Odil Porrua and Kabzinski, Tomasz and Lidschreiber, Michael and Kubíček, Karel and Fořtová, Andrea and Lacroute, Francoise and Vaňáčová, Štěpánka and Cramer, Patrick and Štefl, Richard and Libri, Domenico}, article_location = {CAMBRIDGE}, article_number = {3}, doi = {http://dx.doi.org/10.1016/j.molcel.2014.05.031}, keywords = {CRYPTIC UNSTABLE TRANSCRIPTS; POLYMERASE-II TRANSCRIPTS; BINDING PROTEINS NRD1; QUALITY-CONTROL; SACCHAROMYCES-CEREVISIAE; PERVASIVE TRANSCRIPTION; BIDIRECTIONAL PROMOTERS; POLY(A) POLYMERASE; NUCLEAR EXOSOME; TRAMP COMPLEX}, language = {eng}, issn = {1097-2765}, journal = {Molecular Cell}, title = {Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation}, url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/}, volume = {55}, year = {2014} }
TY - JOUR ID - 1182103 AU - Tudek, Agnieszka - Fuerte, Odil Porrua - Kabzinski, Tomasz - Lidschreiber, Michael - Kubíček, Karel - Fořtová, Andrea - Lacroute, Francoise - Vaňáčová, Štěpánka - Cramer, Patrick - Štefl, Richard - Libri, Domenico PY - 2014 TI - Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation JF - Molecular Cell VL - 55 IS - 3 SP - 467-481 EP - 467-481 PB - CELL PRESS SN - 10972765 KW - CRYPTIC UNSTABLE TRANSCRIPTS KW - POLYMERASE-II TRANSCRIPTS KW - BINDING PROTEINS NRD1 KW - QUALITY-CONTROL KW - SACCHAROMYCES-CEREVISIAE KW - PERVASIVE TRANSCRIPTION KW - BIDIRECTIONAL PROMOTERS KW - POLY(A) POLYMERASE KW - NUCLEAR EXOSOME KW - TRAMP COMPLEX UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/ L2 - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186968/ N2 - The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. We propose that transcription termination and RNA degradation are coordinated by switching between two alternative partners of the NNS complex. ER -
TUDEK, Agnieszka, Odil Porrua FUERTE, Tomasz KABZINSKI, Michael LIDSCHREIBER, Karel KUBÍČEK, Andrea FOŘTOVÁ, Francoise LACROUTE, Štěpánka VAŇÁČOVÁ, Patrick CRAMER, Richard ŠTEFL a Domenico LIBRI. Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation. \textit{Molecular Cell}. CAMBRIDGE: CELL PRESS, roč.~55, č.~3, s.~467-481. ISSN~1097-2765. doi:10.1016/j.molcel.2014.05.031. 2014.
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