TUDEK, Agnieszka, Odil Porrua FUERTE, Tomasz KABZINSKI, Michael LIDSCHREIBER, Karel KUBÍČEK, Andrea FOŘTOVÁ, Francoise LACROUTE, Štěpánka VAŇÁČOVÁ, Patrick CRAMER, Richard ŠTEFL and Domenico LIBRI. Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation. Molecular Cell. CAMBRIDGE: CELL PRESS, 2014, vol. 55, No 3, p. 467-481. ISSN 1097-2765. doi:10.1016/j.molcel.2014.05.031.
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Basic information
Original name Molecular Basis for Coordinating Transcription Termination with Noncoding RNA Degradation
Authors TUDEK, Agnieszka (250 France), Odil Porrua FUERTE (724 Spain), Tomasz KABZINSKI (616 Poland, belonging to the institution), Michael LIDSCHREIBER (276 Germany), Karel KUBÍČEK (203 Czech Republic, belonging to the institution), Andrea FOŘTOVÁ (203 Czech Republic, belonging to the institution), Francoise LACROUTE (250 France), Štěpánka VAŇÁČOVÁ (203 Czech Republic, belonging to the institution), Patrick CRAMER (276 Germany), Richard ŠTEFL (203 Czech Republic, guarantor, belonging to the institution) and Domenico LIBRI (380 Italy).
Edition Molecular Cell, CAMBRIDGE, CELL PRESS, 2014, 1097-2765.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10610 Biophysics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 14.018
RIV identification code RIV/00216224:14740/14:00073613
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.molcel.2014.05.031
UT WoS 000340646600012
Keywords in English CRYPTIC UNSTABLE TRANSCRIPTS; POLYMERASE-II TRANSCRIPTS; BINDING PROTEINS NRD1; QUALITY-CONTROL; SACCHAROMYCES-CEREVISIAE; PERVASIVE TRANSCRIPTION; BIDIRECTIONAL PROMOTERS; POLY(A) POLYMERASE; NUCLEAR EXOSOME; TRAMP COMPLEX
Tags kontrola MP, MP, OA, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 29. 10. 2014 09:37.
Abstract
The Nrd1-Nab3-Sen1 (NNS) complex is essential for controlling pervasive transcription and generating sn/snoRNAs in S. cerevisiae. The NNS complex terminates transcription of noncoding RNA genes and promotes exosome-dependent processing/degradation of the released transcripts. The Trf4-Air2-Mtr4 (TRAMP) complex polyadenylates NNS target RNAs and favors their degradation. NNS-dependent termination and degradation are coupled, but the mechanism underlying this coupling remains enigmatic. Here we provide structural and functional evidence demonstrating that the same domain of Nrd1p interacts with RNA polymerase II and Trf4p in a mutually exclusive manner, thus defining two alternative forms of the NNS complex, one involved in termination and the other in degradation. We show that the Nrd1-Trf4 interaction is required for optimal exosome activity in vivo and for the stimulation of polyadenylation of NNS targets by TRAMP in vitro. We propose that transcription termination and RNA degradation are coordinated by switching between two alternative partners of the NNS complex.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
EE2.3.20.0042, research and development projectName: Internacionalizace programu Strukturní biologie s důrazem na rozvoj nových směrů výzkumu
GA13-18344S, research and development projectName: Studium kódu CTD RNA polymerázy II
Investor: Czech Science Foundation
GBP305/12/G034, research and development projectName: Centrum biologie RNA
084316/Z/07/Z, interní kód MUName: Polyadenylation and mechanisms of nuclear RNA quality control
Investor: Wellcome Trust, Polyadenylation and mechanisms of nuclear RNA quality control
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