The protein inhibitors of peptidases from Eudiplozoon
nipponicum and their preparation in recombinant form by using
the cell-free expression systems

a 2014

The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems

ROUDNICKÝ, Pavel, Jana ILGOVÁ, Milan GELNAR and Martin KAŠNÝ

Basic information

Original name

The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems

Authors

ROUDNICKÝ, Pavel (203 Czech Republic, guarantor, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)

Edition

XXI. Helminthological Days, 2014

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/14:00073622

Organization unit

Faculty of Science

ISBN

978-80-210-6796-7

Keywords in English

inhibitors of peptidases; Eudiplozoon nipponicum; cell-free expression system

Změněno: 12/5/2014 14:33, Mgr. et Mgr. Jana Ilgová, Ph.D.

Abstract

V originále

The cell-free (CF) protein expression systems are useful applications in molecular biology enabling the rapid production of lower amount of selected protein molecule. They represent the alternative to “classical” expression systems adopting the whole live cells, e.g. bacteria - Escherichia coli or yeast – Pichia pastoris. The cell-free expression methods have been used since 1960 and up to date they were significantly improved; a number of commercial CF kits is now available. The proteolytic enzymes – peptidases are essential molecules not only for helminths but also for each living organisms. They are generally participating e.g. in digestive process, in modulation of host immune reaction or in evasion of host immune response. The proteolysis is at the level of the organism well controlled process regulated by number of parasite/hosts specific molecules, including inhibitors – serpins. Number of extra- or intra-cellular serpins was identified from plants, animals, viruses, and microbes. Serpins participate in fundamental physiological processes, such as blood coagulation, fibrinolysis, inflammation, signaling, immune responses and hormone carriage. This suggested their important role in host-parasite interaction. Our work is focused on the characterization of inhibitors (serpins) of serine peptidases which were identified in Eudiplozoon nipponicum (cyprinid fish ectoparasite) transcriptome data.

Links

GAP506/12/1258, research and development project

Name: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Investor: Czech Science Foundation

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

ROUDNICKÝ, Pavel, Jana ILGOVÁ, Milan GELNAR and Martin KAŠNÝ. The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems. In XXI. Helminthological Days. 2014. ISBN 978-80-210-6796-7.

@proceedings{1182537,
   author = {Roudnický, Pavel and Ilgová, Jana and Gelnar, Milan and Kašný, Martin},
   booktitle = {XXI. Helminthological Days},
   keywords = {inhibitors of peptidases; Eudiplozoon nipponicum; cell-free expression system},
   language = {eng},
   isbn = {978-80-210-6796-7},
   title = {The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems},
   year = {2014}
}

TY  - CONF
ID  - 1182537
AU  - Roudnický, Pavel - Ilgová, Jana - Gelnar, Milan - Kašný, Martin
PY  - 2014
TI  - The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems
SN  - 9788021067967
KW  - inhibitors of peptidases
KW  - Eudiplozoon nipponicum
KW  - cell-free expression system
N2  - The cell-free (CF) protein expression systems are useful applications in molecular biology enabling the rapid production of lower amount of selected protein molecule. They represent the alternative to “classical” expression systems adopting the whole live cells, e.g. bacteria - Escherichia coli or yeast – Pichia pastoris. The cell-free expression methods have been used since 1960 and up to date they were significantly improved; a number of commercial CF kits is now available. The proteolytic enzymes – peptidases are essential molecules not only for helminths but also for each living organisms. They are generally participating e.g. in digestive process, in modulation of host immune reaction or in evasion of host immune response. The proteolysis is at the level of the organism well controlled process regulated by number of parasite/hosts specific molecules, including inhibitors – serpins. Number of extra- or intra-cellular serpins was identified from plants, animals, viruses, and microbes. Serpins participate in fundamental physiological processes, such as blood coagulation, fibrinolysis, inflammation, signaling, immune responses and hormone carriage. This suggested their important role in host-parasite interaction. Our work is focused on the characterization of inhibitors (serpins) of serine peptidases which were identified in Eudiplozoon nipponicum (cyprinid fish ectoparasite) transcriptome data.
ER  -

ROUDNICKÝ, Pavel, Jana ILGOVÁ, Milan GELNAR and Martin KAŠNÝ. The protein inhibitors of peptidases from Eudiplozoon nipponicum and their preparation in recombinant form by using the cell-free expression systems. In \textit{XXI. Helminthological Days}. 2014. ISBN~978-80-210-6796-7.

Detailed Information on Publication Record