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@article{1182653,
author = {Bártová, Eva and Foltánková, Veronika and Legartová, Soňa and Sehnalová, Petra and Sorokin, Dmitry and Suchánková, Jana and Kozubek, Stanislav},
article_number = {3},
doi = {http://dx.doi.org/10.4161/nucl.29229},
keywords = {DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin},
language = {eng},
issn = {1949-1034},
journal = {Nucleus},
title = {Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies},
volume = {5},
year = {2014}
}
TY - JOUR
ID - 1182653
AU - Bártová, Eva - Foltánková, Veronika - Legartová, Soňa - Sehnalová, Petra - Sorokin, Dmitry - Suchánková, Jana - Kozubek, Stanislav
PY - 2014
TI - Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies
JF - Nucleus
VL - 5
IS - 3
SP - 269-277
EP - 269-277
PB - Landes Bioscience
SN - 19491034
KW - DNA repair
KW - chromatin
KW - nucleus
KW - nucleolus
KW - Cajal bodies
KW - coilin
N2 - Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.
ER -
BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN, Jana SUCHÁNKOVÁ and Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. \textit{Nucleus}. Landes Bioscience, vol.~5, No~3, p.~269-277. ISSN~1949-1034. doi:10.4161/nucl.29229. 2014.
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