Coilin is rapidly recruited to UVA-induced DNA lesions and
gamma-radiation affects localized movement of Cajal bodies

J 2014

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN et. al.

Basic information

Original name

Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies

Authors

BÁRTOVÁ, Eva (203 Czech Republic), Veronika FOLTÁNKOVÁ (203 Czech Republic), Soňa LEGARTOVÁ (703 Slovakia), Petra SEHNALOVÁ (203 Czech Republic), Dmitry SOROKIN (643 Russian Federation, guarantor, belonging to the institution), Jana SUCHÁNKOVÁ (203 Czech Republic) and Stanislav KOZUBEK (203 Czech Republic)

Edition

Nucleus, Landes Bioscience, 2014, 1949-1034

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.033

RIV identification code

RIV/00216224:14330/14:00073626

Organization unit

Faculty of Informatics

DOI

http://dx.doi.org/10.4161/nucl.29229

UT WoS

000341641600009

Keywords in English

DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin

Abstract

V originále

Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.

Links

CZ.1.07/2.3.00/30.0030, interní kód MU

Name: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministry of Education, Youth and Sports of the CR, 2.3 Human resources in research and development

GBP302/12/G157, research and development project

Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

Citovat

BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN, Jana SUCHÁNKOVÁ and Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. Nucleus. Landes Bioscience, 2014, vol. 5, No 3, p. 269-277. ISSN 1949-1034. Available from: https://dx.doi.org/10.4161/nucl.29229.

@article{1182653,
   author = {Bártová, Eva and Foltánková, Veronika and Legartová, Soňa and Sehnalová, Petra and Sorokin, Dmitry and Suchánková, Jana and Kozubek, Stanislav},
   article_number = {3},
   doi = {http://dx.doi.org/10.4161/nucl.29229},
   keywords = {DNA repair; chromatin; nucleus; nucleolus; Cajal bodies; coilin},
   language = {eng},
   issn = {1949-1034},
   journal = {Nucleus},
   title = {Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies},
   volume = {5},
   year = {2014}
}

TY  - JOUR
ID  - 1182653
AU  - Bártová, Eva - Foltánková, Veronika - Legartová, Soňa - Sehnalová, Petra - Sorokin, Dmitry - Suchánková, Jana - Kozubek, Stanislav
PY  - 2014
TI  - Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies
JF  - Nucleus
VL  - 5
IS  - 3
SP  - 269-277
EP  - 269-277
PB  - Landes Bioscience
SN  - 19491034
KW  - DNA repair
KW  - chromatin
KW  - nucleus
KW  - nucleolus
KW  - Cajal bodies
KW  - coilin
N2  - Cajal bodies are important nuclear structures containing proteins that preferentially regulate RNA-related metabolism. We investigated the cell-type specific nuclear distribution of Cajal bodies and the level of coilin, a protein of Cajal bodies, in non-irradiated and irradiated human tumor cell lines and embryonic stem (ES) cells. Cajal bodies were localized in different nuclear compartments, including DAPI-poor regions, in the proximity of chromocenters, and adjacent to nucleoli. The number of Cajal bodies per nucleus was cell cycle-dependent, with higher numbers occurring during G2 phase. Human ES cells contained a high coilin level in the nucleoplasm, but coilin-positive Cajal bodies were also identified in nuclei of mouse and human ES cells. Coilin, but not SMN, recognized UVA-induced DNA lesions, which was cell cycle-independent. Treatment with gamma-radiation reduced the localized movement of Cajal bodies in many cell types and GFP-coilin fluorescence recovery after photobleaching was very fast in nucleoplasm in comparison with GFP-coilin recovery in DNA lesions. By contrast, nucleolus-localized coilin displayed very slow fluorescence recovery after photobleaching, which indicates very slow rates of protein diffusion, especially in mouse ES cell nucleoli.
ER  -

BÁRTOVÁ, Eva, Veronika FOLTÁNKOVÁ, Soňa LEGARTOVÁ, Petra SEHNALOVÁ, Dmitry SOROKIN, Jana SUCHÁNKOVÁ and Stanislav KOZUBEK. Coilin is rapidly recruited to UVA-induced DNA lesions and gamma-radiation affects localized movement of Cajal bodies. \textit{Nucleus}. Landes Bioscience, 2014, vol.~5, No~3, p.~269-277. ISSN~1949-1034. Available from: https://dx.doi.org/10.4161/nucl.29229.

Detailed Information on Publication Record