The influence of culture conditions on the identification of
Mycobacterium species by MALDI-TOF MS profiling

J 2014

The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling

BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA et. al.

Basic information

Original name

The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling

Authors

BALÁŽOVÁ, Tereza (203 Czech Republic, belonging to the institution), Jitka MAKOVCOVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Michal SLANÝ (203 Czech Republic), Martin FALDYNA (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

FEMS Microbiology Letters, Hoboken (USA), Elsevier Science, 2014, 0378-1097

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.121

RIV identification code

RIV/00216224:14740/14:00073252

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/1574-6968.12408

UT WoS

000333654500011

Keywords in English

MALDI-TOFMS; mycobacteria profiling; strain differentiation; Mycobacterium phlei; Mycobacterium smegmatis

Abstract

V originále

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

QI101A094, research and development project

Name: Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza

Investor: Ministry of Agriculture of the CR, Sustainable development of agrarian sector

Citovat

BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA and Zbyněk ZDRÁHAL. The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. FEMS Microbiology Letters. Hoboken (USA): Elsevier Science, 2014, vol. 353, No 1, p. 77-84. ISSN 0378-1097. Available from: https://dx.doi.org/10.1111/1574-6968.12408.

@article{1183302,
   author = {Balážová, Tereza and Makovcová, Jitka and Šedo, Ondrej and Slaný, Michal and Faldyna, Martin and Zdráhal, Zbyněk},
   article_location = {Hoboken (USA)},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/1574-6968.12408},
   keywords = {MALDI-TOFMS; mycobacteria profiling; strain differentiation; Mycobacterium phlei; Mycobacterium smegmatis},
   language = {eng},
   issn = {0378-1097},
   journal = {FEMS Microbiology Letters},
   title = {The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf},
   volume = {353},
   year = {2014}
}

TY  - JOUR
ID  - 1183302
AU  - Balážová, Tereza - Makovcová, Jitka - Šedo, Ondrej - Slaný, Michal - Faldyna, Martin - Zdráhal, Zbyněk
PY  - 2014
TI  - The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling
JF  - FEMS Microbiology Letters
VL  - 353
IS  - 1
SP  - 77-84
EP  - 77-84
PB  - Elsevier Science
SN  - 03781097
KW  - MALDI-TOFMS
KW  - mycobacteria profiling
KW  - strain differentiation
KW  - Mycobacterium phlei
KW  - Mycobacterium smegmatis
UR  - http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
N2  - Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.
ER  -

BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA and Zbyněk ZDRÁHAL. The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. \textit{FEMS Microbiology Letters}. Hoboken (USA): Elsevier Science, 2014, vol.~353, No~1, p.~77-84. ISSN~0378-1097. Available from: https://dx.doi.org/10.1111/1574-6968.12408.

Detailed Information on Publication Record