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@article{1183302,
author = {Balážová, Tereza and Makovcová, Jitka and Šedo, Ondrej and Slaný, Michal and Faldyna, Martin and Zdráhal, Zbyněk},
article_location = {Hoboken (USA)},
article_number = {1},
doi = {http://dx.doi.org/10.1111/1574-6968.12408},
keywords = {MALDI-TOFMS; mycobacteria profiling; strain differentiation; Mycobacterium phlei; Mycobacterium smegmatis},
language = {eng},
issn = {0378-1097},
journal = {FEMS Microbiology Letters},
title = {The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling},
url = {http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf},
volume = {353},
year = {2014}
}
TY - JOUR
ID - 1183302
AU - Balážová, Tereza - Makovcová, Jitka - Šedo, Ondrej - Slaný, Michal - Faldyna, Martin - Zdráhal, Zbyněk
PY - 2014
TI - The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling
JF - FEMS Microbiology Letters
VL - 353
IS - 1
SP - 77-84
EP - 77-84
PB - Elsevier Science
SN - 03781097
KW - MALDI-TOFMS
KW - mycobacteria profiling
KW - strain differentiation
KW - Mycobacterium phlei
KW - Mycobacterium smegmatis
UR - http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
L2 - http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
N2 - Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. Optimum culture conditions yielding the highest identification success rate against the BioTyper database (Bruker Daltonics) and permitting the possibility of automatic acquisition of mass spectra were found to be distinct for the two mycobacterial species examined. Similarly, individual changes in growth conditions had diverse effects on the two species. For these reasons, thorough control over cultivation conditions should always be employed to maximize the performance and discriminatory power of MALDI-TOF MS profiling, and cultivation conditions must be optimized separately for individual groups of mycobacterial species/strains.
ER -
BALÁŽOVÁ, Tereza, Jitka MAKOVCOVÁ, Ondrej ŠEDO, Michal SLANÝ, Martin FALDYNA and Zbyněk ZDRÁHAL. The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. \textit{FEMS Microbiology Letters}. Hoboken (USA): Elsevier Science, 2014, vol.~353, No~1, p.~77-84. ISSN~0378-1097. Available from: https://dx.doi.org/10.1111/1574-6968.12408.
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