Adaptive phylogeography: functional divergence between
haemoglobins derived from different glacial refugia in the bank
vole

J 2014

Adaptive phylogeography: functional divergence between haemoglobins derived from different glacial refugia in the bank vole

KOTLÍK, Petr, Silvia MARKOVÁ, Libor VOJTEK, Antonín STRATIL, Vlastimil ŠLECHTA et. al.

Basic information

Original name

Adaptive phylogeography: functional divergence between haemoglobins derived from different glacial refugia in the bank vole

Authors

KOTLÍK, Petr (203 Czech Republic, guarantor), Silvia MARKOVÁ (203 Czech Republic), Libor VOJTEK (203 Czech Republic, belonging to the institution), Antonín STRATIL (203 Czech Republic), Vlastimil ŠLECHTA (203 Czech Republic), Pavel HYRŠL (203 Czech Republic, belonging to the institution) and Jeremy B. SEARLE (840 United States of America)

Edition

Proceedings of the Royal Society B-Biological Sciences, Anglie, Royal Society, 2014, 0962-8452

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.051

RIV identification code

RIV/00216224:14310/14:00075557

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1098/rspb.2014.0021

UT WoS

000336784500010

Keywords in English

adaptation; antioxidative capacity; climate change; cysteine; oxidative stress; redox

Abstract

V originále

Over the years, researchers have used presumptively neutral molecular variation to infer the origins of current species’ distributions in northern latitudes (especially Europe). However, several reported examples of genic and chromosomal replacements suggest that end-glacial colonizations of particular northern areas may have involved genetic input from different source populations at different times, coupled with competition and selection. We investigate the functional consequences of differences between two bank vole (Clethrionomys glareolus) haemoglobins deriving from different glacial refugia, one of which partially replaced the other in Britain during end-glacial climate warming. This allows us to examine their adaptive divergence and hence a possible role of selection in the replacement.We determine the amino acid substitution Ser52Cys in the major expressed b-globin gene as the allelic difference. We use structural modelling to reveal that the protein environment renders the 52Cys thiol a highly reactive functional group and we show its reactivity in vitro. We demonstrate that possessing the reactive thiol in haemoglobin increases the resistance of bank vole erythrocytes to oxidative stress. Our study thus provides striking evidence for physiological differences between products of genic variants that spread at the expense of one another during colonization of an area from different glacial refugia.

Citovat

KOTLÍK, Petr, Silvia MARKOVÁ, Libor VOJTEK, Antonín STRATIL, Vlastimil ŠLECHTA, Pavel HYRŠL and Jeremy B. SEARLE. Adaptive phylogeography: functional divergence between haemoglobins derived from different glacial refugia in the bank vole. Proceedings of the Royal Society B-Biological Sciences. Anglie: Royal Society, 2014, vol. 281, No 1786, p. 1-9. ISSN 0962-8452. Available from: https://dx.doi.org/10.1098/rspb.2014.0021.

@article{1183813,
   author = {Kotlík, Petr and Marková, Silvia and Vojtek, Libor and Stratil, Antonín and Šlechta, Vlastimil and Hyršl, Pavel and Searle, Jeremy B.},
   article_location = {Anglie},
   article_number = {1786},
   doi = {http://dx.doi.org/10.1098/rspb.2014.0021},
   keywords = {adaptation; antioxidative capacity; climate change; cysteine; oxidative stress; redox},
   language = {eng},
   issn = {0962-8452},
   journal = {Proceedings of the Royal Society B-Biological Sciences},
   title = {Adaptive phylogeography: functional divergence between haemoglobins derived from different glacial refugia in the bank vole},
   volume = {281},
   year = {2014}
}

TY  - JOUR
ID  - 1183813
AU  - Kotlík, Petr - Marková, Silvia - Vojtek, Libor - Stratil, Antonín - Šlechta, Vlastimil - Hyršl, Pavel - Searle, Jeremy B.
PY  - 2014
TI  - Adaptive phylogeography: functional divergence between haemoglobins derived from different glacial refugia in the bank vole
JF  - Proceedings of the Royal Society B-Biological Sciences
VL  - 281
IS  - 1786
SP  - 1-9
EP  - 1-9
PB  - Royal Society
SN  - 09628452
KW  - adaptation
KW  - antioxidative capacity
KW  - climate change
KW  - cysteine
KW  - oxidative stress
KW  - redox
N2  - Over the years, researchers have used presumptively neutral molecular variation to infer the origins of current species’ distributions in northern latitudes (especially Europe). However, several reported examples of genic and chromosomal replacements suggest that end-glacial colonizations of particular northern areas may have involved genetic input from different source populations at different times, coupled with competition and selection. We investigate the functional consequences of differences between two bank vole (Clethrionomys glareolus) haemoglobins deriving from different glacial refugia, one of which partially replaced the other in Britain during end-glacial climate warming. This allows us to examine their adaptive divergence and hence a possible role of selection in the replacement.We determine the amino acid substitution Ser52Cys in the major expressed b-globin gene as the allelic difference. We use structural modelling to reveal that the protein environment renders the 52Cys thiol a highly reactive functional group and we show its reactivity in vitro. We demonstrate that possessing the reactive thiol in haemoglobin increases the resistance of bank vole erythrocytes to oxidative stress. Our study thus provides striking evidence for physiological differences between products of genic variants that spread at the expense of one another during colonization of an area from different glacial refugia.
ER  -

KOTLÍK, Petr, Silvia MARKOVÁ, Libor VOJTEK, Antonín STRATIL, Vlastimil ŠLECHTA, Pavel HYRŠL and Jeremy B. SEARLE. Adaptive phylogeography: functional divergence between haemoglobins derived from different glacial refugia in the bank vole. \textit{Proceedings of the Royal Society B-Biological Sciences}. Anglie: Royal Society, 2014, vol.~281, No~1786, p.~1-9. ISSN~0962-8452. Available from: https://dx.doi.org/10.1098/rspb.2014.0021.

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