| VOJTEK, Libor, Pavel DOBEŠ, Ender BÜYÜKGÜZEL, Janne ATOSUO and Pavel HYRŠL. Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph. European Journal of Entomology. 2014, vol. 111, No 3, p. 335-340. ISSN 1210-5759. Available from: https://dx.doi.org/10.14411/eje.2014.045. |
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@article{1183817,
author = {Vojtek, Libor and Dobeš, Pavel and Büyükgüzel, Ender and Atosuo, Janne and Hyršl, Pavel},
article_number = {3},
doi = {http://dx.doi.org/10.14411/eje.2014.045},
keywords = {Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens},
language = {eng},
issn = {1210-5759},
journal = {European Journal of Entomology},
title = {Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph},
volume = {111},
year = {2014}
}
TY - JOUR
ID - 1183817
AU - Vojtek, Libor - Dobeš, Pavel - Büyükgüzel, Ender - Atosuo, Janne - Hyršl, Pavel
PY - 2014
TI - Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph
JF - European Journal of Entomology
VL - 111
IS - 3
SP - 335-340
EP - 335-340
SN - 12105759
KW - Antibacterial activity
KW - antimicrobial peptides
KW - Lepidoptera
KW - bioluminescent bacteria
KW - Bombyx mori
KW - Escherichia coli
KW - Galleria mellonella
KW - Photorhabdus luminescens
N2 - We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. We think it is likely that this method could be widely used for determining antibacterial activity in insects and other invertebrates.
ER -
VOJTEK, Libor, Pavel DOBEŠ, Ender BÜYÜKGÜZEL, Janne ATOSUO and Pavel HYRŠL. Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph. \textit{European Journal of Entomology}. 2014, vol.~111, No~3, p.~335-340. ISSN~1210-5759. Available from: https://dx.doi.org/10.14411/eje.2014.045.
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