2014
Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph
VOJTEK, Libor; Pavel DOBEŠ; Ender BÜYÜKGÜZEL; Janne ATOSUO; Pavel HYRŠL et. al.Basic information
Original name
Bioluminescent assay for evaluating antimicrobial activity in insect haemolymph
Authors
VOJTEK, Libor (203 Czech Republic, belonging to the institution); Pavel DOBEŠ (203 Czech Republic, belonging to the institution); Ender BÜYÜKGÜZEL (792 Turkey); Janne ATOSUO (246 Finland) and Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution)
Edition
European Journal of Entomology, 2014, 1210-5759
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
30102 Immunology
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 0.975
RIV identification code
RIV/00216224:14310/14:00073253
Organization unit
Faculty of Science
UT WoS
000343664400004
EID Scopus
2-s2.0-84904608455
Keywords (in Czech)
Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens
Keywords in English
Antibacterial activity; antimicrobial peptides; Lepidoptera; bioluminescent bacteria; Bombyx mori; Escherichia coli; Galleria mellonella; Photorhabdus luminescens
Tags
International impact, Reviewed
Changed: 18/5/2015 13:42, Mgr. Pavel Dobeš, Ph.D.
Abstract
V originále
We describe an antibacterial assay based on bioluminescence of two Gram negative bacteria, Photorhabdus luminescens and transformed Escherichia coli, which can be used as a real-time measurement of antibacterial activity in insect haemolymph. This method is based on the production of the bioluminescence signal depending on the viability of bacterial cells. We observed a significant rapid dose-dependent decrease in bioluminescence using both bacterial species, and Bombyx mori or Galleria mellonella haemolymph, which was confirmed by the decrease in bacterial viability determined by plating. The humoral origin of the antibacterial activity observed in whole haemolymph was confirmed for haemolymph plasma without haemocytes. Antibacterial activity directed against Gram negative bacteria was recorded in unaffected insect larvae as well as after septic injury; increased antibacterial activity of haemolymph was detected in the latter case confirming the inducibility of antimicrobial agents. We think it is likely that this method could be widely used for determining antibacterial activity in insects and other invertebrates.
Links
| EE2.3.30.0009, research and development project |
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| GP206/09/P470, research and development project |
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| QJ1210047, research and development project |
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