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@article{1183882,
author = {Kubáň, Petr and Ďurč, Pavol and Bittová, Miroslava and Foret, František},
article_location = {Amsterdam},
article_number = {January},
doi = {http://dx.doi.org/10.1016/j.chroma.2013.12.039},
keywords = {Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection},
url = {https://doi.org/10.1016/j.chroma.2013.12.039},
volume = {1325},
year = {2014}
}
TY - JOUR
ID - 1183882
AU - Kubáň, Petr - Ďurč, Pavol - Bittová, Miroslava - Foret, František
PY - 2014
TI - Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection
JF - Journal of Chromatography A
VL - 1325
IS - January
SP - 241-246
EP - 241-246
PB - Elsevier
SN - 00219673
KW - Capillary electrophoresis
KW - Contactless conductivity detection
KW - Toxic metabolites
KW - Methanol intoxication
KW - Ethylene glycol intoxication
KW - Body fluid samples
UR - https://doi.org/10.1016/j.chroma.2013.12.039
L2 - https://doi.org/10.1016/j.chroma.2013.12.039
N2 - A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved.
ER -
KUBÁŇ, Petr, Pavol ĎURČ, Miroslava BITTOVÁ a František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, roč.~1325, January, s.~241-246. ISSN~0021-9673. doi:10.1016/j.chroma.2013.12.039. 2014.
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