KUBÁŇ, Petr, Pavol ĎURČ, Miroslava BITTOVÁ and František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. Journal of Chromatography A. Amsterdam: Elsevier, 2014, vol. 1325, January, p. 241-246. ISSN 0021-9673. doi:10.1016/j.chroma.2013.12.039.
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Basic information
Original name Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection
Authors KUBÁŇ, Petr (203 Czech Republic, guarantor, belonging to the institution), Pavol ĎURČ (703 Slovakia, belonging to the institution), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution) and František FORET (203 Czech Republic, belonging to the institution).
Edition Journal of Chromatography A, Amsterdam, Elsevier, 2014, 0021-9673.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 4.169
RIV identification code RIV/00216224:14310/14:00073642
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.chroma.2013.12.039
UT WoS 000330256200031
Keywords in English Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples
Tags AKR, podil, rivok
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 4. 10. 2019 13:22.
A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved.
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
GA13-21919S, research and development projectName: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.
Investor: Czech Science Foundation
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