Separation of oxalate, formate and glycolate in human body
fluid samples by capillary electrophoresis with contactless
conductometric detection

J 2014

Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection

KUBÁŇ, Petr, Pavol ĎURČ, Miroslava BITTOVÁ and František FORET

Basic information

Original name

Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection

Authors

KUBÁŇ, Petr (203 Czech Republic, guarantor, belonging to the institution), Pavol ĎURČ (703 Slovakia, belonging to the institution), Miroslava BITTOVÁ (203 Czech Republic, belonging to the institution) and František FORET (203 Czech Republic, belonging to the institution)

Edition

Journal of Chromatography A, Amsterdam, Elsevier, 2014, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.169

RIV identification code

RIV/00216224:14310/14:00073642

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chroma.2013.12.039

UT WoS

000330256200031

Keywords in English

Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples

Abstract

V originále

A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GA13-21919S, research and development project

Name: Vývoj neinvazivních metod odběru vzorků a analýzy v point-of-care dignostice biologických materiálů.

Investor: Czech Science Foundation

Files attached

ZVV_2014_258_1183882_Separation_of_oxalate.pdf

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Citovat

KUBÁŇ, Petr, Pavol ĎURČ, Miroslava BITTOVÁ and František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. Journal of Chromatography A. Amsterdam: Elsevier, 2014, vol. 1325, January, p. 241-246. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2013.12.039.

@article{1183882,
   author = {Kubáň, Petr and Ďurč, Pavol and Bittová, Miroslava and Foret, František},
   article_location = {Amsterdam},
   article_number = {January},
   doi = {http://dx.doi.org/10.1016/j.chroma.2013.12.039},
   keywords = {Capillary electrophoresis; Contactless conductivity detection; Toxic metabolites; Methanol intoxication; Ethylene glycol intoxication; Body fluid samples},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection},
   url = {https://doi.org/10.1016/j.chroma.2013.12.039},
   volume = {1325},
   year = {2014}
}

TY  - JOUR
ID  - 1183882
AU  - Kubáň, Petr - Ďurč, Pavol - Bittová, Miroslava - Foret, František
PY  - 2014
TI  - Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection
JF  - Journal of Chromatography A
VL  - 1325
IS  - January
SP  - 241-246
EP  - 241-246
PB  - Elsevier
SN  - 00219673
KW  - Capillary electrophoresis
KW  - Contactless conductivity detection
KW  - Toxic metabolites
KW  - Methanol intoxication
KW  - Ethylene glycol intoxication
KW  - Body fluid samples
UR  - https://doi.org/10.1016/j.chroma.2013.12.039
L2  - https://doi.org/10.1016/j.chroma.2013.12.039
N2  - A new method for rapid determination of toxic metabolites after methanol and ethylene glycol intoxication - oxalate, formate and glycolate in various body fluid samples (blood serum, saliva, urine, exhaled breath condensate) by capillary electrophoresis with contactless conductometric detection was developed. A selective separation of the three target analytes from other constituents present in the analyzed biological matrices was achieved in less than 6 min in a fused silica capillary of 25 mu m I.D. using an electrolyte comprising 50 mM L-histidine and 50 mM 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The only sample preparation was dilution with deionized water. The limits of detection were 0.4, 0.6 and 1.3 mu M and limits of quantitation 1.3, 1.9 and 4.2 mu M for oxalate, formate and glycolate, respectively. The method provides a simple and rapid diagnostic test in suspected intoxication and is able to distinguish the ingested liquid, based on its metabolite trace. The method presents a fast screening tool that can be applicable in clinical practice. (C) 2013 Elsevier B.V. All rights reserved.
ER  -

KUBÁŇ, Petr, Pavol ĎURČ, Miroslava BITTOVÁ and František FORET. Separation of oxalate, formate and glycolate in human body fluid samples by capillary electrophoresis with contactless conductometric detection. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2014, vol.~1325, January, p.~241-246. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2013.12.039.

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